Consiglio Nazionale delle Ricerche (CNR), Institute of Biosciences and Bioresources (IBBR), Portici, Italy; CREA Research Centre for Vegetable and Ornamental Crops, Pontecagnano, Italy.
University of Ljubljana, Biotechnical Faculty, Ljubljana, Slovenia.
Trends Plant Sci. 2023 Oct;28(10):1144-1165. doi: 10.1016/j.tplants.2023.05.012. Epub 2023 Jun 16.
The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.
CRISPR/Cas 基因组编辑系统的发现彻底改变了我们对植物基因组的理解。十年来，CRISPR/Cas 一直被用于修饰植物基因组，以研究特定基因和生物合成途径，并加快许多植物物种的育种进程，包括模式植物和非模式作物。尽管 CRISPR/Cas 系统在基因组编辑方面非常高效，但许多瓶颈和挑战减缓了进一步的改进和应用。在本文中，我们讨论了在组织培养、转化、再生和突变体检测过程中可能出现的问题。我们还回顾了新的 CRISPR 平台提供的机会，以及与基因调控、非生物和生物胁迫响应改善以及植物从头驯化相关的特定应用。
