de Souza Leandro Menezes, de Oliveira Isabelle Dias, Sales Flávia Cristina Silva, da Costa Antonio Charlys, Campos Karoline Rodrigues, Abbud Adriano, Guerra Juliana Mariotti, Dos Santos Cirqueira Borges Cinthya, Takahashi Carlos Pires Fernandes Júnior, de Araújo Leonardo José Tadeu
Centro de Patologia, Instituto Adolfo Lutz, Sao Paulo, Brazil.
Programa de Pós Graduação em Ciências da Saúde do Instituto de Assistência Médica ao Servidor Público Estadual - IAMSPE, Sao Paulo, Brazil.
J Genet Eng Biotechnol. 2023 Aug 29;21(1):88. doi: 10.1186/s43141-023-00536-3.
New-generation sequencing (NGS) techniques have brought the opportunity for genomic monitoring of several microorganisms potentially relevant to public health. The establishment of different methods with different mechanisms provides a wide choice, taking into account several aspects. With that in mind, the present aim of the study was to compare basic genomic sequencing metrics that could potentially impact genotyping by nanopores from Oxford Nanopore Technologies and by synthesis from Illumina in clinical samples positive for Chikungunya (CHIKV). Among the metrics studied, running time, read production, and Q score were better represented in Illumina sequencing, while the MinIOn platform showed better response time and greater diversity of generated files. That said, it was possible to establish differences between the studied metrics in addition to verifying that the distinctions in the methods did not impact the identification of the CHIKV virus genotype.
新一代测序（NGS）技术为对几种可能与公共卫生相关的微生物进行基因组监测带来了机遇。考虑到多个方面，建立具有不同机制的不同方法提供了广泛的选择。鉴于此，本研究的当前目的是比较一些基本的基因组测序指标，这些指标可能会影响来自牛津纳米孔技术公司的纳米孔测序以及Illumina公司的合成测序对基孔肯雅病毒（CHIKV）阳性临床样本的基因分型。在所研究的指标中，运行时间、读数产量和Q值在Illumina测序中表现得更好，而MinIOn平台显示出更好的响应时间和生成文件的多样性更高。也就是说，除了验证方法上的差异不会影响基孔肯雅病毒基因型的鉴定外，还能够确定所研究指标之间的差异。
