Islam Md S, VanderLaan Donald, Hickman Josie, Emelianov Stanislav, Dickson Robert M
School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
School of Electrical & Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Photoacoustics. 2023 Jul 7;32:100529. doi: 10.1016/j.pacs.2023.100529. eCollection 2023 Aug.
Synchronously Amplified Photoacoustic Image Recovery (SAPhIRe) offers improved background suppression using non-linear properties of modulatable contrast agents. Using SAPhIRe, multiple contrast agents in the same absorption window can be detected independently based on their unique triplet-state lifetimes. Here, we have demonstrated the unmixing of rose bengal and eosin Y signals from solution based on triplet-state lifetime mapping using both fluorescence and photoacoustics. Varying the pump-probe delay enables resolution and recovery of fast-decaying rose bengal and of slowly decaying eosin Y modulated photoacoustic signals, resulting from optically gated triplet state residence. Distinct images were reconstructed within tissue-mimicking phantom using the fitting coefficients of triplet-state lifetimes. Fluorescence was used to screen for modulation prior to photoacoustic imaging. The results suggest that lifetime unmixing can be utilized to simultaneously detect multiple pathologies with overlapping spectra using photoacoustic imaging.
同步放大光声图像恢复（SAPhIRe）利用可调制造影剂的非线性特性实现了更好的背景抑制。使用SAPhIRe，基于其独特的三重态寿命，可以独立检测处于同一吸收窗口的多种造影剂。在此，我们展示了基于三重态寿命映射，利用荧光和光声技术从溶液中分离孟加拉玫瑰红和伊红Y信号。改变泵浦 - 探测延迟能够分辨和恢复快速衰减的孟加拉玫瑰红以及缓慢衰减的伊红Y调制光声信号，这些信号源于光学门控的三重态驻留。利用三重态寿命的拟合系数，在仿组织体模内重建了不同的图像。在光声成像之前，利用荧光筛选调制情况。结果表明，寿命解混可用于通过光声成像同时检测具有重叠光谱的多种病变。
