Ashifa Nisha, Viswanathan Krishnan, Srinivasan Sivapragasam, Kumar Senthil, Sundaram Rajasekar, Pavithran Varsha K
Department of Periodontology, Rajah Muthiah Dental College & Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
Department of Public Health Dentistry, Rajah Muthiah Dental College & Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
J Adv Periodontol Implant Dent. 2023 May 27;15(1):3-9. doi: 10.34172/japid.2023.009. eCollection 2023.
Sclerostin, a glycoprotein, plays a key role in regulating bone mass. In this study, sclerostin levels in the gingival crevicular fluid (GCF) were assessed in patients with Stage III Grade C generalized periodontitis (SIII-GC) and Stage III Grade B generalized periodontitis (SIII-GB).
This cross-sectional study included 30 participants divided equally into three groups: group I (gingival health), group II (SIII-GC), and group III (SIII-GB). Clinical periodontal parameters like plaque index (PI), gingival bleeding index (GBI), probing pocket depth (PD), and clinical attachment level (CAL) were recorded. A sandwich ELISA was used to determine the sclerostin levels in GCF samples. One-way ANOVA and post hoc Tukey tests were used to analyze the clinical parameters and GCF sclerostin levels. The association between GCF sclerostin levels and periodontal parameters was assessed using Pearson's correlation coefficient (r).
Patients in groups II and III had much higher sclerostin levels in their GCF than in group I (≤0.05). In contrast, no significant difference in sclerostin levels was observed between the two diseased conditions (=0.841). Concerning periodontal parameters, a statistically significant difference was observed between the three groups. There was a positive correlation between the periodontal clinical parameters and the expression levels of sclerostin in GCF (≤0.05).
Increased expression of sclerostin in GCF in patients with periodontitis indicated that it could be considered a reliable biomarker of periodontal disease activity.
硬化蛋白是一种糖蛋白，在调节骨量方面起关键作用。在本研究中，对III期C级广泛性牙周炎（SIII-GC）和III期B级广泛性牙周炎（SIII-GB）患者龈沟液（GCF）中的硬化蛋白水平进行了评估。
这项横断面研究纳入了30名参与者，平均分为三组：第一组（牙龈健康）、第二组（SIII-GC）和第三组（SIII-GB）。记录了菌斑指数（PI）、牙龈出血指数（GBI）、探诊深度（PD）和临床附着水平（CAL）等临床牙周参数。采用夹心酶联免疫吸附测定法（ELISA）测定GCF样本中的硬化蛋白水平。使用单因素方差分析和事后Tukey检验分析临床参数和GCF硬化蛋白水平。使用Pearson相关系数（r）评估GCF硬化蛋白水平与牙周参数之间的关联。
第二组和第三组患者GCF中的硬化蛋白水平远高于第一组（P≤0.05）。相比之下，两种疾病状态下的硬化蛋白水平未观察到显著差异（P=0.841）。关于牙周参数，三组之间观察到统计学上的显著差异。牙周临床参数与GCF中硬化蛋白的表达水平之间存在正相关（P≤0.05）。
牙周炎患者GCF中硬化蛋白表达增加表明，它可被视为牙周疾病活动的可靠生物标志物。
