Ekhlasmand Kermani Mehdi, Kheiri Aida, Amid Reza, Torshabi Maryam, Houshmand Behzad, Parsayan Sepideh
Department of Periodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Adv Periodontol Implant Dent. 2023 Apr 11;15(1):15-21. doi: 10.34172/japid.2023.007. eCollection 2023.
Xenograft and allograft bone substitutes are widely used to replace the missing bone in defects. Since removing the packaging of these grafts can nullify their sterilization, this study aimed to evaluate the sterility and bioactivity changes of an allograft and a xenograft following uncapping/recap.
Two types of commercial allograft and xenograft vials were unpacked and further exposed to operating room air, where implant surgery was performed for one second, ten minutes, and one hour. After three repetitions, samples were analyzed using microbiological tests and scanning electron microscopy (SEM) with energy dispersive x-ray analysis (EDX) for sterility and bioactivity evaluation.
None of the bone graft samples showed microbial growth or bioactivity-negative changes after seven days of unpacking the vials.
Despite the positive results of this study, future studies and more analysis considering influential factors are required. Also, disinfection and air exchange must still be observed during biomaterial application and bone grafting procedures.
异种移植和同种异体移植骨替代物被广泛用于替代缺损中缺失的骨。由于去除这些移植物的包装会使其灭菌失效，本研究旨在评估同种异体移植物和异种移植物在开封/重新封盖后的无菌性和生物活性变化。
打开两种商用同种异体移植物和异种移植物小瓶，并进一步暴露于手术室空气中，在那里进行植入手术一秒、十分钟和一小时。重复三次后，使用微生物学测试以及带有能量色散X射线分析（EDX）的扫描电子显微镜（SEM）对样品进行分析，以评估无菌性和生物活性。
打开小瓶七天后，所有骨移植样品均未显示微生物生长或生物活性阴性变化。
尽管本研究结果为阳性，但仍需要考虑影响因素的未来研究和更多分析。此外，在生物材料应用和骨移植过程中仍必须注意消毒和空气交换。
