Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Laboratory of Se-enriched Food Development, Ankang 725000, China.
J Agric Food Chem. 2023 Sep 13;71(36):13363-13375. doi: 10.1021/acs.jafc.3c04193. Epub 2023 Aug 30.
The objective of the current study was to explore the potential mechanism of Ziyang selenium-enriched green tea polysaccharide (Se-GTP) against obesity. The results showed that Se-GTP significantly alleviated obesity and related metabolic disorders caused by high-fat diet (HFD) in mice. 16S rRNA gene sequencing results revealed that Se-GTP improved gut microbiota disturbance of obese mice and facilitated proliferation of probiotics such as , , , and . In addition, the colonic content of succinate, a product of microbial metabolite in connection with adipocyte thermogenesis, was significantly enhanced by Se-GTP treatment. Therefore, Se-GTP facilitated brown adipose tissue (BAT) thermogenesis and inguinal white adipose tissue (iWAT) browning in obese mice, which could be revealed by increased expressions of thermogenic marker proteins UCP1, PGC-1α, and CIDEA in BAT and iWAT. Interestingly, Se-GTP intervention also observably increased the content of M2-like macrophages in iWAT of obese mice. To summarize, the results of this study are the first to show that Se-GTP can stimulate the browning of iWAT and BAT thermogenesis to counteract obesity, which may be pertinent with the alteration of gut microbiota in obese mice.
本研究旨在探讨紫阳富硒绿茶多糖（Se-GTP）防治肥胖的潜在机制。结果表明，Se-GTP 可显著减轻高脂饮食（HFD）诱导的肥胖小鼠肥胖及其相关代谢紊乱。16S rRNA 基因测序结果显示，Se-GTP 改善了肥胖小鼠的肠道微生物失调，促进了 、 、 、 等益生菌的增殖。此外，Se-GTP 处理还显著增加了与脂肪细胞产热相关的微生物代谢产物琥珀酸的结肠含量。因此，Se-GTP 促进了肥胖小鼠棕色脂肪组织（BAT）和腹股沟白色脂肪组织（iWAT）的产热，BAT 和 iWAT 中解偶联蛋白 1（UCP1）、过氧化物酶体增殖物激活受体 γ 共激活因子 1α（PGC-1α）和细胞死亡诱导因子（CIDEA）等产热标志物蛋白的表达增加证实了这一点。有趣的是，Se-GTP 干预还明显增加了肥胖小鼠 iWAT 中 M2 样巨噬细胞的含量。综上所述，本研究结果首次表明，Se-GTP 可刺激 iWAT 和 BAT 产热，从而防治肥胖，这可能与肥胖小鼠肠道微生物的改变有关。
