Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Food Funct. 2021 Sep 20;12(18):8467-8477. doi: 10.1039/d1fo01361k.
Activating the thermogenic function of adipocytes is an attractive therapeutic strategy against obesity and its associated metabolic complications. Proanthocyanidins are a class of polyphenols which are widely found in plants and daily foods. This aim of this study is to investigate the modulatory effects of grape seed proanthocyanidin extract (GSPE) on brown adipose tissue (BAT) activity, browning of white adipose tissue (WAT) and microbiome regulation in high-fat diet (HFD)-fed mice and its associated molecular mechanism. An 8-week administration of GSPE at 200 mg per kg bw in mice significantly reduced their final body weight, antagonized their HFD-induced insulin resistance and elevated their levels of adiponectin and leptin, respectively ( < 0.05). GSPE significantly increased the expression levels of thermogenic marker UCP1 in BAT and elevated the expression of a key transcription factor of browning, PRDM16, and thermogenic markers UCP1 and PGC-1α in inguinal white adipose tissue (iWAT). The high doses of GSPE also increased the levels of acetic acid, propionic acid and butyric acid in the colon of HFD-fed mice ( < 0.05). Furthermore, GSPE normalized the colonic / ratios, reversed the relative abundance of , , , and induced by HFD, and improved the structural diversity of the gut microbiota in C57BL/6J mice.
激活脂肪细胞的产热功能是治疗肥胖及其相关代谢并发症的一种有吸引力的治疗策略。原花青素是一类广泛存在于植物和日常食物中的多酚。本研究旨在研究葡萄籽原花青素提取物（GSPE）对高脂肪饮食（HFD）喂养小鼠棕色脂肪组织（BAT）活性、白色脂肪组织（WAT）褐变和微生物组调节的调节作用及其相关分子机制。在小鼠中，GSPE 以 200mg/kg bw 的剂量连续给药 8 周，可显著降低其最终体重，拮抗 HFD 诱导的胰岛素抵抗，并分别升高其脂联素和瘦素水平（<0.05）。GSPE 显著增加了 BAT 中产热标志物 UCP1 的表达水平，并上调了腹股沟白色脂肪组织（iWAT）中褐变关键转录因子 PRDM16 以及产热标志物 UCP1 和 PGC-1α 的表达。高剂量的 GSPE 还增加了 HFD 喂养小鼠结肠中乙酸、丙酸和丁酸的水平（<0.05）。此外，GSPE 使结肠/比值正常化，逆转了 HFD 诱导的相对丰度、、、和 ，并改善了 C57BL/6J 小鼠肠道微生物组的结构多样性。
