外源喷施4-氯苯氧乙酸钠盐促进桑叶生长和黄酮类化合物生物合成。
Exogenous spraying of 4-chlorophenoxyacetic acid sodium salt promotes growth and flavonoid biosynthesis of mulberry leaves ( L.).
作者信息
Zhang Zhen, Duan Yun, Zhang Yu, Zhang Shanying, Liang Xiaoyu, Li Jiahao, Ren Saihao, Zhang Chenghui, Wang Meng
机构信息
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101 China.
School of Food Science and Engineering, Hainan University, Haikou, 570228 China.
出版信息
Physiol Mol Biol Plants. 2023 Jul;29(7):971-983. doi: 10.1007/s12298-023-01339-z. Epub 2023 Jul 31.
UNLABELLED
Mulberry ( L.) leaves are known as an ideal vegetable with good antioxidant effect, which can bring delicious taste and multiple health benefits. In the present study, the effects of 4-Chlorophenoxyacetic acid sodium salt (4-CPANa) treatment on growth and content of flavonoid compounds in mulberry leaves were investigated. Moreover, the changes in the expression levels of genes involved in flavonoid biosynthetic pathways, and the accumulation of important secondary metabolites including rutin (Rut), chlorogenic acid (ChA), isoquercitrin (IQ) and astragalin (Ast), were investigated in mulberry leaves. The results showed that 4-CPANa treatment could significantly promote the differentiation and growth of mulberry, increased shoot number, bud number, leaf fresh weight and leaf area of mulberry compared with control. Besides, the contents of ChA, Rut, IQ and Ast were significantly increased after 4-CPANa (5 mg/L) treatment. Further analysis revealed that 5 mg/L 4-CPANa strongly induced the expression of flavonoid biosynthesis-related genes including flavonoid 3--glucosyltransferase (F3GT) gene, chalcone synthase (CHS) gene, 4-xoumarate-CoA ligase (4CL) and phenylalanine ammonia lyase (PAL) gene. In conclusion, exogenous spraying of 4-CPANa provides a new way to improve the medicinal quality and development of mulberry leaf food with high value.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-023-01339-z.
未标注
桑叶是一种具有良好抗氧化作用的理想蔬菜，能带来美味口感并具有多种健康益处。在本研究中，研究了4-氯苯氧乙酸钠盐（4-CPANa）处理对桑叶生长和黄酮类化合物含量的影响。此外，还研究了桑叶中黄酮生物合成途径相关基因表达水平的变化，以及包括芦丁（Rut）、绿原酸（ChA）、异槲皮苷（IQ）和紫云英苷（Ast）在内的重要次生代谢产物的积累。结果表明，与对照相比，4-CPANa处理能显著促进桑树的分化和生长，增加桑树的新梢数、芽数、叶片鲜重和叶面积。此外，4-CPANa（5 mg/L）处理后，ChA、Rut、IQ和Ast的含量显著增加。进一步分析表明，5 mg/L 4-CPANa强烈诱导黄酮生物合成相关基因的表达，包括黄酮3-O-葡萄糖基转移酶（F3GT）基因、查尔酮合酶（CHS）基因、4-香豆酸辅酶A连接酶（4CL）和苯丙氨酸解氨酶（PAL）基因。总之，外源喷施4-CPANa为提高桑叶药用品质和开发高价值桑叶食品提供了一种新途径。
补充信息
在线版本包含可在10.1007/s12298-023-01339-z获取的补充材料。
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