桑叶膳食补充剂通过提高猪模型中瘦素刺激的脂肪分解来部分减少脂肪量。
Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model.
机构信息
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, Shaanxi, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Sciences and Technologies, Northwest A&F University, Yangling, Shaanxi, China.
出版信息
J Ethnopharmacol. 2020 Mar 1;249:112416. doi: 10.1016/j.jep.2019.112416. Epub 2019 Nov 19.
ETHNOPHARMACOLOGICAL RELEVANCE
Mulberry leaves are the dry leaves of Morus nigra L. trees, which are widely cultivated in central and southern China. Mulberry has a long history of medicinal use, such as anti-stress, lowering blood glucose and anti-obesity.
AIM OF THE STUDY
Explore the effects of mulberry leaves on fat deposition as well as the underlying mechanisms.
MATERIALS AND METHODS
Total of 48 fattening pigs weighing about 70 kg were randomly allotted to normal diet or die supplemented with 5% (w/w) mulberry leave powder. Changes of fat mass, indicated by backfat thickness was measured with Piggyback tester, blood triglyceride and cholesterol were tested using commercial biochemical kits, serum hormones were estimated by ELISA, and leptin-related signaling activity were assessed using western-blot.
RESULTS
Supplementation with Mulberry leaf feed (MF) significantly reduced serum triglyceride and free cholesterol concentrations and increased the ratio of high-density lipoprotein cholesterol (HDL-c) to low-density lipoprotein cholesterol (LDL-c), while serum glucose and free fatty acids remained unchanged. Dietary MF resulted in a significant reduction in the size of adipocytes and backfat thickness (P < 0.05). Accordingly, hormone-sensitive lipase (HSL) in backfat was significantly up-regulated and fatty acid synthase (FAS) was down-regulated by MF supplementation (both P < 0.05). Furthermore, MF supplementation significantly elevated circulating leptin and adiponectin without influencing serum insulin and glucocorticoid. Moreover, significantly higher leptin receptor (Leptin-R) and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) were detected in MF-supplemented pigs, suggesting an enhanced leptin signaling induced by MF in the subcutaneous fat.
CONCLUSIONS
Mulberry leaves have obvious anti-obesity effects, providing a theoretical basis for the development of mulberry leaves as a drug against obesity.
民族药理学相关性
桑叶为桑科植物桑 Morus nigra L. 的干燥叶，在中国中部和南部广泛种植。桑叶具有悠久的药用历史，如抗应激、降血糖和抗肥胖。
研究目的
探讨桑叶对脂肪沉积的影响及其潜在机制。
材料和方法
共 48 头体重约 70kg 的育肥猪随机分为正常饮食组或添加 5%（w/w）桑叶粉的饮食组。使用 Piggyback 测试仪测量背膘厚度的变化，以反映脂肪量的变化；使用商业生化试剂盒检测血清三酰甘油和胆固醇；使用 ELISA 法估计血清激素；使用 Western blot 法评估瘦素相关信号活性。
结果
桑叶饲料（MF）补充显著降低血清三酰甘油和游离胆固醇浓度，增加高密度脂蛋白胆固醇（HDL-c）与低密度脂蛋白胆固醇（LDL-c）的比值，而血清葡萄糖和游离脂肪酸保持不变。饲粮 MF 导致脂肪细胞和背膘厚度显著减小（P<0.05）。相应地，MF 补充显著上调背膘组织中的激素敏感脂肪酶（HSL），下调脂肪酸合成酶（FAS）（均 P<0.05）。此外，MF 补充显著提高循环瘦素和脂联素水平，而不影响血清胰岛素和糖皮质激素。此外，MF 补充组的瘦素受体（Leptin-R）和磷酸化信号转导和转录激活因子 3（p-STAT3）明显升高，表明 MF 可增强皮下脂肪中的瘦素信号。
结论
桑叶具有明显的抗肥胖作用，为桑叶作为肥胖药物的开发提供了理论依据。
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