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基于对口腔细菌抑制活性筛选热灭活乳酸菌以及热灭活CP3365口服对健康参与者牙周健康影响的双盲、随机、安慰剂对照试验。

Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial.

作者信息

Sakata Shinji, Sakamaki Yukiko, Yuki Masahiro, Sugaya Tsutomu, Hirota Tatsuhiko

机构信息

Core Technology Laboratories, Asahi Quality & Innovations, Ltd, Moriya-Shi, Ibaraki, Japan.

Periodontology & Endodontology Department of Oral Health Science Faculty of Dental Medicine, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.

出版信息

J Oral Microbiol. 2023 Aug 28;15(1):2250649. doi: 10.1080/20002297.2023.2250649. eCollection 2023.

DOI:10.1080/20002297.2023.2250649
PMID:37649969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10464545/
Abstract

OBJECTIVES

The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants.

MATERIALS AND METHODS

An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum. The effects of HKL administration on various parameters (plaque control record, bleeding on probing, and probing pocket depth) were assessed in a randomized, placebo-controlled trial. Participants in the test and placebo groups ( = 32) consumed oral tablets containing placebo or HKL daily for 8 weeks. Oral bacteria in supra-plaque and saliva were identified using 16S rRNA gene community profiling analysis.

RESULTS

Heat-killed CP3365 significantly ( < 0.05) decreased the viability of oral bacteria and was selected for clinical trials. Administration of HKL CP3365 significantly ( < 0.05) inhibited increases in each parameter. No changes in the relative abundance of P. gingivalis or F. nucleatum subsp. nucleatum were detected by HKL CP3365, but the relative abundance of oral bacteria (genera Porphyromonas, Fusobacterium, and Haemophilus) was significantly ( < 0.05) decreased.

CONCLUSION

HKL CP3365 effectively inhibited oral bacteria growth and was useful for maintaining periodontal health.

CLINICAL TRIAL REGISTRATION

[https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045656].

摘要

目的

本研究旨在筛选具有抗生素活性的热灭活乳酸菌（HKL），并研究该菌对健康受试者牙周参数的维持效果。

材料与方法

进行体外评估以检测乳酸菌对牙龈卟啉单胞菌和具核梭杆菌具核亚种的抑制效果。在一项随机、安慰剂对照试验中评估了HKL给药对各项参数（菌斑控制记录、探诊出血和探诊深度）的影响。试验组和安慰剂组的参与者（每组 = 32人）连续8周每日服用含安慰剂或HKL的口服片剂。使用16S rRNA基因群落分析对龈上菌斑和唾液中的口腔细菌进行鉴定。

结果

热灭活的CP3365显著（P < 0.05）降低了口腔细菌的活力，并被选入临床试验。HKL CP3365给药显著（P < 0.05）抑制了各项参数的增加。HKL CP3365未检测到牙龈卟啉单胞菌或具核梭杆菌具核亚种相对丰度的变化，但口腔细菌（卟啉单胞菌属、梭杆菌属和嗜血杆菌属）的相对丰度显著（P < 0.05）降低。

结论

HKL CP3365有效抑制口腔细菌生长，有助于维持牙周健康。

临床试验注册

[https://www.umin.ac.jp/ctr/index.htm]，标识符[UMIN000045656]

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/126c1d21d0c7/ZJOM_A_2250649_F0007_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/61e14cab81b0/ZJOM_A_2250649_F0001_B.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/24a119868e60/ZJOM_A_2250649_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/126c1d21d0c7/ZJOM_A_2250649_F0007_B.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/5874f211b9c8/ZJOM_A_2250649_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/24a119868e60/ZJOM_A_2250649_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f7/10464545/126c1d21d0c7/ZJOM_A_2250649_F0007_B.jpg

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