Department of Mechanical Engineering, National Taipei University of Technology, Taipei, 106, Taiwan; Institute of Mechatronic Engineering, National Taipei University of Technology, Taipei, 106, Taiwan.
Institute of Mechatronic Engineering, National Taipei University of Technology, Taipei, 106, Taiwan; Taiwan Semiconductor Manufacturing Company, Hsinchu, 30078, Taiwan.
Talanta. 2024 Jan 1;266(Pt 2):125096. doi: 10.1016/j.talanta.2023.125096. Epub 2023 Aug 26.
In this study, a graphene electrochemical sensor based on laser graphene polymer material was proposed to induce graphene formation on polyimide substrates via fiber laser. The laser produces stable power and results to achieve the benefits of consistency, conductivity, and flexibility. The electrochemical three-electrodes were manufactured on polyimide to replace the traditional three-electrodes by achieving small size and portability. An electrode activation is the modification of laser-scribed graphene electrodes (LSG) to facilitate the binding of liver cancer sites. The evaluation is performed by differential pulse Voltammetry (DPV) to detect cancer proteins in the phosphate buffer saline (PBS) buffer and serum. In a serum environment, the concentrations of alpha-fetoprotein (AFP) and Carcinoembryonic antigen (CEA) were detected from 0.75 ng ml to 100 ng ml, AFP and CEA electrodes have a good linear range (R = 0.96 and R = 0.98), indicating the sensor's sensitivity and specificity for cancer detection. In addition, two types of carcinogenic proteins were monitored in the PBS and successfully detected in this experiment. Based on the results, the appropriate LSG sensor may be used for monitoring with limited resources. Electrode manufacturing is simple, fast, low-cost, small in size, convenient to carry, stable, instant detection, and flexible.
在本研究中，提出了一种基于激光石墨烯聚合物材料的石墨烯电化学传感器，通过光纤激光在聚酰亚胺衬底上诱导石墨烯形成。激光产生稳定的功率，实现了一致性、导电性和灵活性的优势。电化学三电极在聚酰亚胺上制造，以通过实现小尺寸和便携性来代替传统的三电极。电极激活是激光刻蚀石墨烯电极（LSG）的修饰，以促进肝癌部位的结合。通过差分脉冲伏安法（DPV）进行评估，以在磷酸盐缓冲盐水（PBS）缓冲液和血清中检测癌症蛋白。在血清环境中，从 0.75ng/ml 到 100ng/ml 检测到甲胎蛋白（AFP）和癌胚抗原（CEA）的浓度，AFP 和 CEA 电极具有良好的线性范围（R=0.96 和 R=0.98），表明传感器对癌症检测的灵敏度和特异性。此外，在 PBS 中监测了两种致癌蛋白，并在该实验中成功检测到。基于这些结果，适当的 LSG 传感器可能用于有限资源的监测。电极制造简单、快速、低成本、体积小、携带方便、稳定、即时检测、灵活。
