Diao Huaqiong, Li Yiming, Sun Wenjun, Zhang Jing, Wang Min, Chen Yufei, Zhou Fen, Li Xiaoli
Department of Encephalopathy, Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China.
Department of Chinese Medicine, Zibo Central Hospital, Shandong, China.
Behav Brain Res. 2023 Oct 2;454:114652. doi: 10.1016/j.bbr.2023.114652. Epub 2023 Aug 29.
The modified multi-platform method (MMPM) is used to induce animal models of paradoxical sleep deprivation and impairs memory in rodents. However, variations in MMPM protocols have contributed to inconsistent conclusions across studies. This meta-analysis aimed to assess the variations of the MMPM and their effects on memory in rats and mice. A comprehensive search identified 60 studies, and 50 were included in our meta-analysis. Overall, the meta-analysis showed that the MMPM significantly reduced the percentage of time spent in target quadrants (I = 54 %, 95 % confidence interval [CI] = [-1.83, -1.18]) and the number of platform-area crossings (I = 26 %, 95 % CI = [-1.71, -1.07]) in the Morris water maze (MWM) and shortened the latency to entering the dark compartment in the passive avoidance task (I = 68 %, 95 % CI = [-1.36, -0.57]), but it increased the number of errors in the radial arm water maze (RAWM) (I = 59 %, 95 % CI = [1.29, 2.07]). Additionally, mice performed worse on the MWM, whereas rats performed worse on the passive avoidance task. More significant memory deficits were found in cross-learning and post-learning MMPM in the MWM and RAWM, respectively. This study provided evidence that the MMPM can be used in preclinical studies of memory deficits induced by paradoxical sleep deprivation.
改良多平台法（MMPM）用于诱导矛盾性睡眠剥夺的动物模型，并损害啮齿动物的记忆。然而，MMPM方案的差异导致了各研究结果不一致。本荟萃分析旨在评估MMPM的差异及其对大鼠和小鼠记忆的影响。全面检索后确定了60项研究，其中50项纳入了我们的荟萃分析。总体而言，荟萃分析表明，MMPM显著降低了在莫里斯水迷宫（MWM）中目标象限停留时间的百分比（I = 54%，95%置信区间[CI] = [-1.83, -1.18]）和穿越平台区域的次数（I = 26%，95% CI = [-1.71, -1.07]），并缩短了被动回避任务中进入暗室的潜伏期（I = 68%，95% CI = [-1.36, -0.57]），但增加了放射状臂水迷宫（RAWM）中的错误次数（I = 59%，95% CI = [1.29, 2.07]）。此外，小鼠在MWM上表现较差，而大鼠在被动回避任务上表现较差。在MWM和RAWM中，分别在交叉学习和学习后MMPM中发现了更显著的记忆缺陷。本研究提供了证据表明MMPM可用于矛盾性睡眠剥夺所致记忆缺陷的临床前研究。
