多杀性巴氏杆菌和胸膜肺炎放线杆菌毒素对猪肺泡巨噬细胞的影响。
Effect of Pasteurella multocida and Haemophilus pleuropneumoniae toxins on swine alveolar macrophages.
作者信息
Pijoan C
出版信息
Vet Immunol Immunopathol. 1986 Sep;13(1-2):141-9. doi: 10.1016/0165-2427(86)90055-3.
Supernatants were obtained from 18 hr. broth cultures of Pasteurella multocida strains D82 and D62 (serotype D, toxigenic), Kobe 6 (type D, non-toxigenic), A50 and X73 (type A, non-toxigenic), Haemophilus pleuropneumoniae serotypes 1, 5 and 6, Haemophilus sp. taxon "minor group" (2 strains) and an avirulent serotype 1 strain of H. pleuropneumoniae. The supernates were filtered, pH-neutralized and tested for cytotoxicity after incubation for 18 hours in the presence of swine alveolar macrophage monolayers. Supernatants from H. pleuropneumoniae serotypes 1, 5 and 6 were cytocidal.
从多杀性巴氏杆菌菌株D82和D62（血清型D，产毒素）、神户6（D型，不产毒素）、A50和X73（A型，不产毒素）、胸膜肺炎放线杆菌血清型1、5和6、嗜血杆菌属“小群”（2株）以及胸膜肺炎放线杆菌无毒血清型1菌株的18小时肉汤培养物中获取上清液。将上清液过滤、pH值中和，并在猪肺泡巨噬细胞单层存在的情况下孵育18小时后测试其细胞毒性。来自胸膜肺炎放线杆菌血清型1、5和6的上清液具有杀细胞作用。
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