Rapp V J, Ross R F, Young T F
J Clin Microbiol. 1985 Dec;22(6):945-50. doi: 10.1128/jcm.22.6.945-950.1985.
Of 30 sows from a herd believed to be free of Haemophilus pleuropneumoniae infection, 2 had complement-fixing antibodies to H. pleuropneumoniae serotype 5. Necropsy and microbiological examination of the two sows revealed no evidence of H. pleuropneumoniae infection; however, Haemophilus taxon "minor group" and a urease-negative, indole-positive Haemophilus sp. were isolated from numerous respiratory tract sites in both sows. Isolation of these Haemophilus spp. was facilitated by serially diluting specimens in two broth media. Pigs from a closed, respiratory disease-free herd were inoculated with four strains of Haemophilus taxon "minor group" to determine whether the organism induces antibodies which cross-react with H. pleuropneumoniae in the complement fixation test. Antigenic heterogeneity among the taxon "minor group" strains was apparent; however, antibodies cross-reacting between these strains and H. pleuropneumoniae serotypes 1 through 5 and 7 were not detected.
在一个据信无胸膜肺炎放线杆菌感染的猪群中的30头母猪里，有2头对胸膜肺炎放线杆菌血清型5具有补体结合抗体。对这两头母猪进行尸检和微生物学检查，未发现胸膜肺炎放线杆菌感染的证据；然而，在这两头母猪的多个呼吸道部位分离出了嗜血杆菌属“次要菌群”以及一株脲酶阴性、吲哚阳性的嗜血杆菌。通过在两种肉汤培养基中对标本进行连续稀释，有助于分离出这些嗜血杆菌属细菌。用四株嗜血杆菌属“次要菌群”菌株接种来自一个封闭的、无呼吸道疾病猪群的猪，以确定该微生物是否会诱导产生在补体结合试验中与胸膜肺炎放线杆菌发生交叉反应的抗体。“次要菌群”菌株之间的抗原异质性很明显；然而，未检测到这些菌株与胸膜肺炎放线杆菌血清型1至5和7之间的交叉反应抗体。
