Yang W C, Schultz R D
Vet Immunol Immunopathol. 1986 Sep;13(1-2):19-29. doi: 10.1016/0165-2427(86)90045-0.
The effects of prednisolone sodium succinate on the responses of porcine lymphocytes to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) were investigated. Sensitivity of peripheral blood lymphocytes (PBL) to the synthetic glucocorticoid, prednisolone, was related to age of the lymphocyte donor. The greatest sensitivity was found in PBL from animals less than one week old; PBL from animals between 2 to 4 months retained some glucocorticoid sensitive cells; whereas, PBL from animals older than 6 months were exceptionally resistant to steroid. Similar age-associated sensitivities were found for lymphocytes from lymph node, spleen and thymus. Significant differential sensitivities among the various lymphoid organs were found with the thymic lymphocyte possessing the greatest sensitivity to steroid and the PBL lymph node and splenic lymphocytes possessing the highest resistance to the suppressive effects of steroid. The age related differences in sensitivity to steroid did not appear to be caused by differences in the number of steroid receptors because lymphocytes from susceptible and resistant animals had similar numbers of receptors. The results suggest that the age related sensitivity may be associated with a higher percentage of sensitive thymic-derived lymphocyte in the PBL, lymph node and spleen of the younger animals. Results of this study also suggest that the adult pig (6 months) should be classified as a steroid resistant species.
研究了琥珀酸钠泼尼松龙对猪淋巴细胞对植物血凝素（PHA）和商陆有丝分裂原（PWM）反应的影响。外周血淋巴细胞（PBL）对合成糖皮质激素泼尼松龙的敏感性与淋巴细胞供体的年龄有关。在小于1周龄动物的PBL中发现最大敏感性；2至4个月龄动物的PBL保留了一些糖皮质激素敏感细胞；而6个月龄以上动物的PBL对类固醇具有极高的抗性。在来自淋巴结、脾脏和胸腺的淋巴细胞中也发现了类似的年龄相关敏感性。在各种淋巴器官之间发现了显著的差异敏感性，其中胸腺淋巴细胞对类固醇具有最大敏感性，而PBL、淋巴结和脾淋巴细胞对类固醇的抑制作用具有最高抗性。对类固醇敏感性的年龄相关差异似乎不是由类固醇受体数量的差异引起的，因为来自易感和抗性动物的淋巴细胞具有相似数量的受体。结果表明，年龄相关敏感性可能与较年幼动物的PBL、淋巴结和脾脏中较高比例的敏感胸腺来源淋巴细胞有关。本研究结果还表明，成年猪（6个月）应归类为类固醇抗性物种。
