Pedersen Kathrine, Rasmussen Rikke Kongsgaard, Dittrich Anita, Lauridsen Henrik
Department of Clinical Medicine (Comparative Medicine Lab), Aarhus University, 8200, Aarhus N, Denmark.
BMC Res Notes. 2021 Apr 28;14(1):157. doi: 10.1186/s13104-021-05574-z.
Cardiac regeneration in the axolotl has been found to rely on the innate immune system, and especially macrophages have been demonstrated to play a vital role in regulating the regenerative process. In this study we wanted to induce a pro- and anti-inflammatory milieu in the axolotl during heart regeneration to test the resilience of the regenerative response.
This was induced via repeated intrapericardial injections of lipopolysaccharide or prednisolone during a 40-day regeneration period in order to challenge the presumably fine-tuned inflammatory response that normally facilitates regeneration. We observed a local and systemic leucocyte response to pro- and anti-inflammatory stimulation, but we found cardiac regeneration to be structurally and functionally unaffected.
已发现美西螈的心脏再生依赖于先天免疫系统，尤其是巨噬细胞已被证明在调节再生过程中发挥着至关重要的作用。在本研究中，我们希望在心脏再生期间在美西螈体内诱导促炎和抗炎环境，以测试再生反应的恢复力。
在40天的再生期内，通过反复心包内注射脂多糖或泼尼松龙来诱导这种情况，以挑战通常促进再生的可能经过精细调节的炎症反应。我们观察到局部和全身白细胞对促炎和抗炎刺激的反应，但我们发现心脏再生在结构和功能上未受影响。
