Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Vet Med Sci. 2023 Nov;9(6):2731-2738. doi: 10.1002/vms3.1250. Epub 2023 Sep 1.
Medicinal plants or their products are popular substitutes for antibiotics or other synthetic growth promoters for their beneficial effects and cost-effectiveness. Clove (Cv) and tulsi (Ts) are rich in phytochemicals and are used to augment the growth performance of poultry.
The current investigation was carried out to evaluate the effects of Cv and Ts supplementation in drinking water on the morphologic and morphometric adaptations in the lymphoid organs.
The experiment was conducted on 60 broiler chicks, divided into 4 homogenous groups (5 pens/group and 3 broilers/pen) named T0 (control), T1 (0.5% Cv and 2% Ts), T2 (1% Cv and 3% Ts) and T3 (1.5% Cv and 4% Ts). The treatments were administered from days 8 to 28. Lymphoid organs (thymus, spleen and bursa of Fabricius) were collected on days 14, 21 and 28 after sacrificing five broilers from each group.
The treatment groups had higher thymus and spleen weights on day 21, whereas the weights of the bursa of Fabricius were higher on days 14, 21 and 28. In the histomorphologic study, no noticeable difference in the histoarchitectures of the lymphoid organs was noticed. However, substantial differences in the cortico-medullary ratio and the number of follicles in the thymus and bursa of Fabricius, respectively, were found in the histomorphometric investigation.
The inclusion of Cv and Ts in drinking water augments the weight of lymphoid organs with histomorphometric adaptations that might improve the immune status in broilers.
药用植物或其产品因其有益的效果和成本效益，是抗生素或其他合成生长促进剂的热门替代品。丁香（Cv）和圣罗勒（Ts）富含植物化学物质，用于提高家禽的生长性能。
本研究旨在评估丁香和圣罗勒在饮用水中的补充对淋巴器官形态和形态计量适应性的影响。
本实验在 60 只肉鸡上进行，分为 4 个同质组（每组 5 个围栏，每个围栏 3 只肉鸡），分别命名为 T0（对照）、T1（0.5%丁香和 2%圣罗勒）、T2（1%丁香和 3%圣罗勒）和 T3（1.5%丁香和 4%圣罗勒）。从第 8 天到第 28 天，对鸡进行处理。在处死每组的 5 只鸡后，于第 14、21 和 28 天收集淋巴器官（胸腺、脾脏和法氏囊）。
处理组在第 21 天的胸腺和脾脏重量较高，而法氏囊的重量在第 14、21 和 28 天较高。在组织形态学研究中，未观察到淋巴器官的组织结构有明显差异。然而，在组织形态计量学研究中，发现胸腺和法氏囊的皮质-髓质比和滤泡数量有显著差异。
在饮用水中添加丁香和圣罗勒可增加淋巴器官的重量，具有形态计量学适应性，可能改善肉鸡的免疫状态。
