Bukrinskaia A G, Tentsov Iu Iu, Vorkunova G K, Tabachnikov B I, Tiasto E A
Vopr Virusol. 1986 May-Jun;31(3):280-3.
The results of influenza diagnosis during the outbreak of 1985 are presented. Nasopharyngeal secretions from 94 patients were examined by virus isolation in chick embryos, fluorescent antibody technique (FAT). enzyme-immunoassay (EIA), and dot-blot hybridization method (DBHM). The virus was isolated in 28%, FAT was positive in 22%, EIA in 47% of the cases. Among 94 secretion specimens 40 were tested by DBHM. In this instance, virus was isolated in 37%, EIA was positive in 65%, and DBHM in 85% of the cases. It seems advisable to use EIA based on the detection of the type-specific antigen (matrix protein) and DBHM which identifies the serovariant of influenza virus.
本文呈现了1985年流感爆发期间的诊断结果。对94例患者的鼻咽分泌物采用鸡胚病毒分离法、荧光抗体技术（FAT）、酶免疫测定法（EIA）和斑点印迹杂交法（DBHM）进行检测。28%的病例病毒分离成功，FAT阳性率为22%，EIA阳性率为47%。94份分泌物标本中的40份采用DBHM检测。在此情况下，病毒分离成功率为37%，EIA阳性率为65%，DBHM阳性率为85%。基于检测型特异性抗原（基质蛋白）的EIA和鉴定流感病毒血清变异株的DBHM似乎是可取的。
