Ivanov N B, Zhemkov V P, Gromov V I, Cherkasov A N, Shashkov L A
Vopr Virusol. 1986 Jul-Aug;31(4):404-9.
The process of microfiltration carried out in the regimen of destruction of concentrational polarization layer decreases considerably the retention of admixture proteins in filtrational concentration of virus suspension. This allows the membrane technology to be used not only for concentration but also for profound purification of the virus-containing allantoic fluid for preparation of inactivated influenza virus. Using the microfiltration method, vaccine preparations of any influenza virus strain may be obtained without loss of biological activity. The resultant vaccine is highly immunogenic and safe for man. The filtration technology makes it possible to restore the preparations discarded because of low virus titres or excess protein content.
在浓差极化层破坏模式下进行的微滤过程，能显著降低病毒悬液过滤浓缩过程中杂质蛋白的截留率。这使得膜技术不仅可用于浓缩，还可用于对含病毒尿囊液进行深度纯化，以制备灭活流感病毒。采用微滤方法，可获得任何流感病毒株的疫苗制剂，且不会损失生物活性。所得疫苗对人体具有高度免疫原性且安全。过滤技术还能使因病毒滴度低或蛋白质含量过高而被废弃的制剂得以恢复。
