Gong Jing-Zhi, Fan Yi-Min, Yuan Wanzhe, Pan Ming, Liu Dandan, Tao Jian-Ping, Huang Si-Yang
Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
College of Veterinary Medicine, Hebei Agricultural University, Baoding, PR China.
Vet Parasitol. 2023 Oct;322:110021. doi: 10.1016/j.vetpar.2023.110021. Epub 2023 Aug 29.
Fasciolosis is a widely distributed zoonosis reported over 81 countries around the world. Good and early diagnostic method is critical in controlling this disease and prevention of injury to the liver and bile ducts. In this study, we identified a novel member (cathepsin L7) of cathepsin family from Fasciola spp.. Firstly, the biological character of CL7 was analyzed according to the information of cathepsin L family, and then rCL7 was expressed and purified, a new iELISA based on CL7 was developed. The results exhibited CL7 iELISA had 100% sensitivity 100% specificity in sheep (cut-off 1.329) and 100% sensitivity 93.75% specificity in cattle (cut-off 0.756). Moreover, anti-Fasciola CL7 antibodies could be detected in early Fasciola gigantica infected buffaloes, as early as 3 week-post-infection (WPI). In conclusion, it is suggested that CL7 with low cost, early detection, good specificity and sensitivity could be used as a candidate antigen for detection of ruminant fasciolosis.
肝片吸虫病是一种广泛分布的人畜共患病，全球81个以上国家均有报告。良好且早期的诊断方法对于控制这种疾病以及预防肝脏和胆管损伤至关重要。在本研究中，我们从肝片吸虫属中鉴定出组织蛋白酶家族的一个新成员（组织蛋白酶L7）。首先，根据组织蛋白酶L家族的信息分析了CL7的生物学特性，然后表达并纯化了重组CL7（rCL7），开发了一种基于CL7的新型间接酶联免疫吸附测定法（iELISA）。结果显示，CL7 iELISA在绵羊中（临界值为1.329）具有100%的敏感性和100%的特异性，在牛中（临界值为0.756）具有100%的敏感性和93.75%的特异性。此外，在感染大片吸虫的水牛中，早在感染后3周（WPI）就能检测到抗肝片吸虫CL7抗体。总之，建议成本低、能早期检测、具有良好特异性和敏感性的CL7可作为反刍动物肝片吸虫病检测的候选抗原。
