Tang Y T, Yin S G, Peng C F, Tang J Y, Jia G, Che L Q, Liu G M, Tian G, Chen X L, Cai J Y, Kang B, Zhao H
Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China, Ministry of Agriculture and Rural Affairs of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Poult Sci. 2023 Nov;102(11):103037. doi: 10.1016/j.psj.2023.103037. Epub 2023 Aug 17.
Currently, antimicrobial peptides (AMPs) are of growing interest as potential substitutes for antibiotic growth promoters in animal production. The present study was conducted to evaluate the effects of dietary supplementation of bioengineering artificial Parasin I protein (API) and artificial plectasin protein (APL) (named as compound bioengineering protein, CBP) on growth performance and intestinal health of broilers. A total of 450 one-day-old Arbor Acres male healthy broilers were randomly allotted to 5 dietary groups with 10 replicates of 9 individuals in each replicate and supplemented with 0, 250, 500, 750, and 1,000 mg/kg CBP for 6 wk. Dietary CBP supplementation increased (P < 0.01) body weight (6 wk), average daily gain (0-6 wk), and average daily feed intake (3-6 wk and 0-6 wk). CBP addition enhanced antioxidant capacity, which was accompanied by the higher (P < 0.05) activity of serum total antioxidant capacity (T-AOC) (750 mg/kg), jejunal glutathione peroxidase (750 mg/kg), and T-AOC (500 and 1,000 mg/kg). Dietary CBP addition improved intestinal health, reflecting by the increased (P < 0.05) villus height to crypt depth ratio in the duodenum, the upregulated (P < 0.01) mRNA levels of claudin-1 (500 and 750 mg/kg) in the ileum, the downregulated (P < 0.01) mRNA expression of occludin (500 mg/kg) in the duodenum and claudin-1 (500 mg/kg) and occludin (500 and 750 mg/kg) in the jejunum, and the upregulated mRNA expression of (P < 0.01) mucin2 (MUC2) (1,000 mg/kg) in the duodenum. In addition, CBP upregulated (P < 0.01) IL-10 (1,000 mg/kg) in duodenum and ileum, and downregulated (P < 0.05) the mRNA expression of IL-6 (750 and 1,000 mg/kg), interferon-γ (1,000 mg/kg) in the jejunum and TNF-α (250 mg/kg) in the ileum. Furthermore, dietary CBP increased (P < 0.01) the abundance of total bacteria and Lactobacillus (500 and 750 mg/kg), and reduced (P < 0.05) the abundance of Escherichia coli (750 mg/kg) in the cecum. In conclusion, CBP supplementation enhances the antioxidant capacity, intestinal health, immune function, and ameliorates the gut microflora population, thus improving the growth performance of broilers. Dietary supplementation of 750 mg/kg CBP exhibits a better beneficial effect.
目前，抗菌肽（AMPs）作为动物生产中抗生素生长促进剂的潜在替代品，受到越来越多的关注。本研究旨在评估日粮中添加生物工程人工副溶菌素I蛋白（API）和人工杀甲素蛋白（APL）（命名为复合生物工程蛋白，CBP）对肉鸡生长性能和肠道健康的影响。将450只1日龄健康的艾维茵雄性肉鸡随机分为5个日粮组，每组10个重复，每个重复9只鸡，并分别添加0、250、500、750和1000 mg/kg的CBP，为期6周。日粮中添加CBP可增加（P < 0.01）体重（6周龄时）、平均日增重（0至6周龄）以及平均日采食量（3至6周龄和0至6周龄）。添加CBP可增强抗氧化能力，表现为血清总抗氧化能力（T-AOC）（750 mg/kg）、空肠谷胱甘肽过氧化物酶（750 mg/kg）以及T-AOC（500和1000 mg/kg）的活性更高（P < 0.05）。日粮中添加CBP可改善肠道健康，表现为十二指肠绒毛高度与隐窝深度比值增加（P < 0.05）、回肠中紧密连接蛋白-1（claudin-1）（500和750 mg/kg）的mRNA水平上调（P < 0.01）、十二指肠中闭合蛋白（occludin）（500 mg/kg）以及空肠中claudin-1（500 mg/kg）和occludin（500和750 mg/kg）的mRNA表达下调（P < 0.01），以及十二指肠中粘蛋白2（MUC2）（1000 mg/kg）的mRNA表达上调（P < 0.01）。此外，CBP上调（P < 0.01）十二指肠和回肠中白细胞介素-10（IL-）（1000 mg/kg）的表达，并下调（P < 0.05）空肠中白细胞介素-6（IL-6）（750和1000 mg/kg）、干扰素-γ（1000 mg/kg）以及回肠中肿瘤坏死因子-α（TNF-α）（250 mg/kg）的mRNA表达。此外，日粮中添加CBP可增加（P < 0.01）盲肠中总细菌和乳酸杆菌（500和750 mg/kg）的数量，并减少（P < 0.05）盲肠中大肠杆菌（750 mg/kg）的数量。总之，添加CBP可增强抗氧化能力、改善肠道健康、增强免疫功能并改善肠道微生物群落，从而提高肉鸡生长性能。日粮中添加750 mg/kg CBP表现出更好的有益效果。
