Brinkman M B, van Gend H W, Mattern E M
Z Lebensm Unters Forsch. 1986 Aug;183(2):97-100. doi: 10.1007/BF01041924.
With the described method it is possible to determinate residues of Dapsone (DDS) and its acetylated metabolites (mono- and diacetyldapsone) in milk. The determination is being carried out with a FAST-LC system (Fully Automated Sample Treatment Liquid Chromatography). The purification is a combination of dialysing the milk against water and absorption of the nonpolar components from the dialysate on the preconcentration column of the LC-system used (RP, 40 microns). With back-flush, the concentrate is being injected into the analytical column (RP-8, 5 microns). The detection is being carried out with the help of a UV-absorbance detector, at a wavelength of 296 mm. The detection limit is approximately 2 micrograms/l (DADDS: 5 micrograms/l). The required amount of the sample is 5 ml. The recovery, reproducibility and linearity for the three components in milk are good.
使用所述方法能够测定牛奶中氨苯砜（DDS）及其乙酰化代谢物（单乙酰氨苯砜和二乙酰氨苯砜）的残留量。测定采用FAST-LC系统（全自动样品处理液相色谱法）进行。净化过程是将牛奶与水进行透析，并将透析液中的非极性成分吸附在用的液相色谱系统的预浓缩柱（RP，40微米）上。通过反冲，将浓缩物注入分析柱（RP-8，5微米）。借助紫外吸光检测器在波长296纳米处进行检测。检测限约为2微克/升（二乙酰氨苯砜：5微克/升）。所需样品量为5毫升。牛奶中这三种成分的回收率、重现性和线性良好。
