Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education, Gannan Medical University, University Park in Rongjiang New District, Ganzhou 341000, People's Republic of China; Department of Orthopedics, First Affiliated Hospital of Gannan Medical University, Jinling East Avenue, Zhanggong District, Ganzhou 341000, People's Republic of China.
Department of Infectious Diseases, Ganzhou People's Hospital, 17 Hongqi Avenue, Zhanggong District, Ganzhou 341000, People's Republic of China.
Eur J Pharm Sci. 2023 Nov 1;190:106574. doi: 10.1016/j.ejps.2023.106574. Epub 2023 Sep 1.
Biomimetic nano-platforms have attracted extensive attention due to their good biocompatibility, low immunogenicity, and homologous targeting to lesions. In this study, glioma cell membranes are used to encapsulate indocyanine green (ICG) loaded nanoparticles (SLNP/ICG), termed as SLNP/ICG@M for targeted photodynamic therapy (PDT) against glioma. Cell membrane modification significantly enhances cellular uptake of SLNP/ICG@M in homologous glioma cells in vitro and tumor distribution in vivo. Furthermore, SLNP/ICG@M can stimulate glioma cells to generate plentiful reactive oxygen species (ROS) under NIR irradiation, finally producing excellent photo-cytotoxicity and the optimal tumor growth inhibition with a tumor suppression rate of 93.2%. We also confirm that SLNP/ICG@M combined with NIR irradiation could activate mitochondria mediated apoptosis pathway, and the increased proliferation of CD4 T cells and CD8 T cells accompanied by immune activation further enhances PDT effect of SLNP/ICG@M. Herein, SLNP/ICG@M is a promising biomimetic nano drug delivery system for glioma targeted PDT therapy.
仿生纳米平台由于其良好的生物相容性、低免疫原性和对病变的同源靶向性而受到广泛关注。在这项研究中，使用神经胶质瘤细胞膜包裹负载吲哚菁绿（ICG）的纳米颗粒（SLNP/ICG），称为 SLNP/ICG@M，用于针对神经胶质瘤的靶向光动力疗法（PDT）。细胞膜修饰显著增强了 SLNP/ICG@M 在体外同源神经胶质瘤细胞中的细胞摄取和体内肿瘤分布。此外，SLNP/ICG@M 可以在近红外辐射下刺激神经胶质瘤细胞产生大量活性氧（ROS），最终产生优异的光细胞毒性和最佳的肿瘤生长抑制作用，肿瘤抑制率为 93.2%。我们还证实，SLNP/ICG@M 联合近红外辐射可以激活线粒体介导的细胞凋亡途径，并且伴随着免疫激活的 CD4 T 细胞和 CD8 T 细胞的增殖增加进一步增强了 SLNP/ICG@M 的 PDT 效应。因此，SLNP/ICG@M 是一种有前途的仿生纳米药物递送系统，可用于针对神经胶质瘤的靶向 PDT 治疗。
