Stossi Fabio, Singh Pankaj K, Safari Kazem, Marini Michela, Labate Demetrio, Mancini Michael A
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA; GCC Center for Advanced Microscopy and Image Informatics, Houston, TX, USA.
GCC Center for Advanced Microscopy and Image Informatics, Houston, TX, USA; Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, USA.
Biochem Pharmacol. 2023 Oct;216:115770. doi: 10.1016/j.bcp.2023.115770. Epub 2023 Sep 1.
Measuring single cell responses to the universe of chemicals (drugs, natural products, environmental toxicants etc.) is of paramount importance to human health as phenotypic variability in sensing stimuli is a hallmark of biology that is considered during high throughput screening. One of the ways to approach this problem is via high throughput, microscopy-based assays coupled with multi-dimensional single cell analysis methods. Here, we will summarize some of the efforts in this vast and growing field, focusing on phenotypic screens (e.g., Cell Painting), single cell analytics and quality control, with particular attention to environmental toxicology and drug screening. We will discuss advantages and limitations of high throughput assays with various end points and levels of complexity.
测量单个细胞对各种化学物质（药物、天然产物、环境毒物等）的反应对人类健康至关重要，因为在高通量筛选过程中，感知刺激时的表型变异性是生物学的一个标志。解决这个问题的方法之一是通过基于显微镜的高通量检测结合多维度单细胞分析方法。在这里，我们将总结这个广阔且不断发展的领域中的一些成果，重点关注表型筛选（例如，细胞绘画）、单细胞分析和质量控制，特别关注环境毒理学和药物筛选。我们将讨论具有不同终点和复杂程度的高通量检测的优点和局限性。
