National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, 252 Wu-Hsing St., Taipei 110, Taiwan; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., 7 Sec. 2, Shipai, Beitou, Taipei 112, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan.
Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126669. doi: 10.1016/j.ijbiomac.2023.126669. Epub 2023 Sep 1.
This study compares the bioactivity of six sulfated polysaccharides derived from glucose- and sucrose-feeding extracted from P. cocos. Anti-inflammatory potentials of these polysaccharides were evaluated by pretreating lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. Of the tested polysaccharides, the sulfated polysaccharide derived from sucrose-feeding at the concentration of 40 g/l (referred to as "suc 40") exhibited the highest anti-inflammatory activity, of 83 %, and 33 % inhibition of IL-6 and TNF-α secretion, respetively. It achieved this by inhibiting the p-38 and c-Jun N-terminal kinase (JNK) MAPK signaling pathways. On the other hand, the sulfated polysaccharide derived from glucose-feeding at a concentration of 20 g/l (referred to as "glc 20") demonstrated the greatest anti-lung cancer activity. This was achieved by inducing apoptotic-related molecules, such as poly (ADP-ribose) polymerase (PARP) and CHOP. Furthermore, glc 20 had the highest contents of sulfate, fucose, and mannose compared to the other tested polysaccharides. This suggests that the composition of monosaccharide residues are critical factors influencing the anti-inflammatory and anti-cancer activities of these sulfated polysaccharides. Overall, this study highlights the potential of sulfated polysaccharides derived from P. cocos to function as bioactive compounds with anti-inflammatory and anti-cancer properties.
这项研究比较了六种从葡萄糖和蔗糖喂养的椰子中提取的硫酸化多糖的生物活性。通过预处理脂多糖（LPS）诱导的 RAW264.7 细胞炎症，评估了这些多糖的抗炎潜力。在测试的多糖中，浓度为 40 g/l 的蔗糖喂养衍生的硫酸化多糖（称为“suc 40”）表现出最高的抗炎活性，为 83%，分别抑制 IL-6 和 TNF-α分泌 33%。它通过抑制 p-38 和 c-Jun N 末端激酶（JNK）MAPK 信号通路来实现这一点。另一方面，浓度为 20 g/l 的葡萄糖喂养衍生的硫酸化多糖（称为“glc 20”）表现出最强的抗肺癌活性。这是通过诱导凋亡相关分子，如多聚（ADP-核糖）聚合酶（PARP）和 CHOP 来实现的。此外，与其他测试的多糖相比，glc 20 具有最高的硫酸根、岩藻糖和甘露糖含量。这表明单糖残基的组成是影响这些硫酸化多糖抗炎和抗癌活性的关键因素。总的来说，这项研究强调了来自椰子的硫酸化多糖作为具有抗炎和抗癌特性的生物活性化合物的潜力。
