Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
Int J Biol Macromol. 2020 Aug 15;157:311-318. doi: 10.1016/j.ijbiomac.2020.04.014. Epub 2020 Apr 14.
Poria cocos (Polyporacea), is a fungus used in traditional Chinese medicine. A study of the valuable sulfated polysaccharides (SPS) with the structure and pharmaceutical benefits from the mycelial culture conditions of P. cocos was attempted. The SPSs were fractionated by gel filtration chromatography to give a fucose-containing mannoglucan polysaccharide (denoted as FMGP): The main skeleton was a 1,4-α-Man-interlaced-1,3-β-glucan with interlaced 6-O-α-l-fucosyl 1,4-α-Glc and 1,4-α-Gal branches. FMGP dramatically inhibited cell migration in the highly metastatic human lung cancer cell line CL1-5 cells. Mechanistically, FMGP dramatically downregulated the expression of TGFβRI and inhibited phosphorylation of FAK and AKT. Moreover, FMGP reduced the metastasis-related protein, Slug, expression. This is the first paper reporting a branched 1,3-β-mannoglucan from P. cocos and its anti-lung cancer CL1-5 cells migration activities.
药用真菌茯苓（多孔菌科）是传统中药的重要组成部分。本研究以药用真菌茯苓为研究对象，通过优化其菌丝体发酵条件，获得具有明确结构和生物活性的硫酸化多糖（SPS）。利用凝胶过滤色谱对 SPS 进行分级分离，得到一种含有岩藻糖的甘露葡聚糖多糖（FMGP）：其主链由交替排列的 1,4-α-Man 和 1,3-β-Glc 组成，含有 6-O-α-l-岩藻糖基 1,4-α-Glc 和 1,4-α-Gal 分支。FMGP 能显著抑制高转移性人肺癌细胞系 CL1-5 细胞的迁移。作用机制研究表明，FMGP 能显著下调 TGFβRI 的表达，抑制 FAK 和 AKT 的磷酸化，降低与转移相关的 Slug 蛋白的表达。这是首次报道来源于茯苓的分支 1,3-β-甘露聚糖及其抗肺癌 CL1-5 细胞迁移活性的研究。
