Rahman Sayeedur, Jahan Najeeb
Dept. of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, India.
Dept. of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, India.
J Ayurveda Integr Med. 2021 Jul-Sep;12(3):500-505. doi: 10.1016/j.jaim.2021.04.016. Epub 2021 Aug 6.
Mazaryun (Daphne oleoides Schreb.) is used as an anti-inflammatory drug in Unani medicine after detoxification, as it is defined under fourth-degree drugs.
OBJECTIVE(S): To evaluate and compare the anti-inflammatory activity of crude and detoxified Mazaryun in maximum and minimum doses.
Anti-inflammatory activity was carried out by carrageenan-induced paw edema test. Wistar rats of either sex, weighing 150-200 gm, were divided into seven groups (I, II, IIIA, IIIB, IVA, IVB, and V) of six animals in each. Group I - plain control, administered with 1 ml of 1% CarboxyMethyl Cellulose (CMC); Group II - standard control, given Diclofenac Sodium (6 mg/kg); Group III - crude Mazaryun and Group IV - detoxified Mazaryun, A and B are maximum and minimum doses of test drug, respectively; and V group - positive control was not treated with any other drugs. The data was statistically analyzed by ANOVA repeated for inter-group analysis and ANOVA one-way for intra-group analysis with post hoc Tukey Kramer multiple comparison test. The GC-MS analysis of crude and detoxified leaves of Mazaryun was also carried out in continuation of study to determine the phytochemical changes before and after detoxification.
Maximum dose of detoxified Mazaryun and standard control groups showed significant anti-inflammatory activity at p < 0.001, and detoxified Mazaryun showed dose-dependent activity. The GC-MS fingerprints showed totally eight different chemical constituents in its crude and detoxified form.
The study standardised the concept of detoxication in Unani medicine, as the detoxified Mazaryun showed significant anti-inflammatory activity and present of totally different chemicals constituents.
马扎林（Daphne oleoides Schreb.）在经过解毒处理后，作为一种用于尤那尼医学的抗炎药物，因为它被定义为四级药物。
评估和比较最大剂量和最小剂量的粗制和解毒马扎林的抗炎活性。
通过角叉菜胶诱导的足爪肿胀试验进行抗炎活性研究。选取体重150 - 200克的Wistar大鼠，雌雄不限，分为七组（I、II、IIIA、IIIB、IVA、IVB和V），每组六只动物。第一组为空白对照组，给予1毫升1%羧甲基纤维素（CMC）；第二组为标准对照组，给予双氯芬酸钠（6毫克/千克）；第三组为粗制马扎林，第四组为解毒马扎林，A和B分别为受试药物的最大剂量和最小剂量；第五组为阳性对照组，不给予任何其他药物。数据采用重复测量方差分析进行组间分析，采用单因素方差分析进行组内分析，并进行事后Tukey Kramer多重比较检验。为了确定解毒前后的植物化学变化，还继续对马扎林的粗叶和解毒叶进行了气相色谱 - 质谱分析。
解毒马扎林的最大剂量组和标准对照组在p < 0.001时显示出显著的抗炎活性，且解毒马扎林呈现出剂量依赖性活性。气相色谱 - 质谱指纹图谱显示其粗制和解毒形式共有八种不同的化学成分。
该研究规范了尤那尼医学中的解毒概念，因为解毒后的马扎林显示出显著的抗炎活性，且存在完全不同的化学成分。
