Atasever-Arslan Belkis, Yilancioglu Kaan, Bekaroglu Maide G, Taskin Emre, Altinoz Eyup, Cetiner Selim
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
Gen Physiol Biophys. 2015 Apr;34(2):201-7. doi: 10.4149/gpb_2014034. Epub 2015 Feb 12.
Cytotoxic effects of essential oils extracted from Dunaliella salina on SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determination of the composition of essential oils found in Dunaliella salina extract. All experimented concentrations of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than the tested concentrations of the extract on ECV304 human endothelial cells used as a control. Fifthy compounds were detected in GC-MS analysis of the extract, and five major compounds were predominantly found as follows: octadecanoic acid, methyl ester (27.43%); hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39%); octadecanoic acid (5.03%), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells might be due to high concentrations of octadecanoic acid and hexadecanoic acid. Furthermore, results indicate that the extract demonstrates some proliferative effect on ECV304 cells in a dose-dependent manner between 0.25 and 5 μg/ml. These results suggest that Dunaliella salina may have anticancer potential against human neuroblastoma cells.
本研究调查了盐生杜氏藻提取的精油对SH-SY5Y人神经母细胞瘤细胞的细胞毒性作用。采用气相色谱-质谱联用（GC-MS）分析来确定盐生杜氏藻提取物中精油的成分。盐生杜氏藻提取物对SH-SY5Y人神经母细胞瘤细胞的所有实验浓度，其细胞毒性均显著高于作为对照的提取物对ECV304人内皮细胞的测试浓度。在提取物的GC-MS分析中检测到50种化合物，主要发现了5种主要化合物，如下所示：十八烷酸甲酯（27.43%）；十六烷酸甲酯（棕榈酸甲酯，24.82%）；9,12,15-十八碳三烯酸乙酯，(Z,Z,Z)-（7.39%）；十八烷酸（5.03%），十五烷酸（3.60%）。盐生杜氏藻提取物对SH-SY5Y人神经母细胞瘤细胞的细胞毒性活性可能归因于高浓度的十八烷酸和十六烷酸。此外，结果表明提取物在0.25至5μg/ml之间对ECV304细胞表现出一定的剂量依赖性增殖作用。这些结果表明盐生杜氏藻可能对人神经母细胞瘤细胞具有抗癌潜力。
