Ali Muddassar, Salma Umme, Khan Irfan Amir, Ahmad Taseer, Bashir Kashif, Khan Taous, Mahnashi Mater H, Alhasaniah Abdulaziz H, Al Awadh Ahmed A, Almazni Ibrahim A, Alshahrani Mohammed M, Shah Abdul Jabbar
Cardiovascular Research Group, Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 22060 KPK, Pakistan.
Department of Pharmacology, College of Pharmacy, University of Sargodha, University Road, Sargodha, Punjab 40100, Pakistan.
Biomed Res Int. 2022 Sep 6;2022:7011789. doi: 10.1155/2022/7011789. eCollection 2022.
Stapf ex Haines () fruit is used traditionally in the treatment of respiratory illnesses including asthma. However, there is no scientific evidence supporting its antiasthmatic activity. The current study was conducted to evaluate its antiasthmatic effects using and approaches. The methanolic crude extract of fruit (Co.Cr.) was used and antiasthmatic activity was carried out using ovalbumin- (OVA-) sensitized and OVA-challenged BALB/c mice. In bronchorelaxant activity of crude extract, aqueous and n-hexane fractions of were carried out on isolated rat tracheal strips. Co.Cr. (200 and 400 mg/kg) attenuated ovalbumin-induced changes in lung histochemistry with % decrease in peribronchial inflammation of 14.1 ± 0.21 and 65.8 ± 0.22 and % decrease in total inflammatory cell count of 35.7 ± 2.80 and 53.3 ± 2.30 in bronchoalveolar lavage fluid. Co.Cr., aqueous, and n-hexane fraction of attenuated the precontractions induced by high K (80 mM) and carbachol (1 M), respectively. In conclusion, the results showed that possesses antiasthmatic activity via relaxant effect on bronchial smooth muscle which is mediated through calcium channel blockade and antimuscarinic activity. This study provides scientific evidence of the traditional use of in the management of allergic asthma.
海恩斯氏（Stapf ex Haines）果实传统上用于治疗包括哮喘在内的呼吸道疾病。然而，尚无科学证据支持其抗哮喘活性。本研究旨在使用[具体方法1]和[具体方法2]方法评估其抗哮喘作用。使用了海恩斯氏果实的甲醇粗提物（Co.Cr.），并采用卵清蛋白（OVA）致敏和OVA激发的BALB/c小鼠进行抗哮喘活性研究。在粗提物的支气管舒张活性研究中，对分离的大鼠气管条进行了海恩斯氏果实的水相和正己烷馏分的实验。Co.Cr.（200和400mg/kg）减轻了卵清蛋白诱导的肺组织化学变化，支气管周围炎症减少百分比分别为14.1±0.21和65.8±0.22，支气管肺泡灌洗液中总炎症细胞计数减少百分比分别为35.7±2.80和53.3±2.30。Co.Cr.、海恩斯氏果实的水相和正己烷馏分分别减轻了高钾（80mM）和卡巴胆碱（1μM）诱导的预收缩。总之，结果表明海恩斯氏果实通过对支气管平滑肌的舒张作用具有抗哮喘活性，这是通过钙通道阻滞和抗毒蕈碱活性介导的。本研究为海恩斯氏果实在过敏性哮喘管理中的传统应用提供了科学证据。
