Blood-brain barrier water exchange measurements using contrast-enhanced ASL.

A technique for quantifying regional blood-brain barrier (BBB) water exchange rates using contrast-enhanced arterial spin labelling (CE-ASL) is presented and evaluated in simulations and in vivo. The two-compartment ASL model describes the water exchange rate from blood to tissue, , but to estimate in practice it is necessary to separate the intra- and extravascular signals. This is challenging in standard ASL data owing to the small difference in values. Here, a gadolinium-based contrast agent is used to increase this difference and enable the signal components to be disentangled. The optimal post-contrast blood ( ) at 3 T was determined in a sensitivity analysis, and the accuracy and precision of the method quantified using Monte Carlo simulations. Proof-of-concept data were acquired in six healthy volunteers (five female, age range 24-46 years). The sensitivity analysis identified the optimal at 3 T as 0.8 s. Simulations showed that could be estimated in individual cortical regions with a relative error % and coefficient of variation %; however, a high dependence on blood was also observed. In volunteer data, mean parameter values in grey matter were: arterial transit time  s, cerebral blood flow  mL blood/min/100 mL tissue and water exchange rate s . CE-ASL can provide regional BBB water exchange rate estimates; however, the clinical utility of the technique is dependent on the achievable accuracy of measured values.