Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, V6T 1Z1, Canada.
Graduate Program in Bioinformatics, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
Nat Commun. 2023 Sep 4;14(1):5380. doi: 10.1038/s41467-023-40907-5.
Anaerobic digestion of municipal mixed sludge produces methane that can be converted into renewable natural gas. To improve economics of this microbial mediated process, metabolic interactions catalyzing biomass conversion to energy need to be identified. Here, we present a two-year time series associating microbial metabolism and physicochemistry in a full-scale wastewater treatment plant. By creating a co-occurrence network with thousands of time-resolved microbial populations from over 100 samples spanning four operating configurations, known and novel microbial consortia with potential to drive methane production were identified. Interactions between these populations were further resolved in relation to specific process configurations by mapping metagenome assembled genomes and cognate gene expression data onto the network. Prominent interactions included transcriptionally active Methanolinea methanogens and syntrophic benzoate oxidizing Syntrophorhabdus, as well as a Methanoregulaceae population and putative syntrophic acetate oxidizing bacteria affiliated with Bateroidetes (Tenuifilaceae) expressing the glycine cleavage bypass of the Wood-Ljungdahl pathway.
城市混合污泥的厌氧消化会产生甲烷，甲烷可转化为可再生天然气。为了提高该微生物介导过程的经济性，需要确定催化生物质转化为能源的代谢相互作用。在这里，我们展示了一项为期两年的时间序列研究，该研究将微生物代谢与一个全规模污水处理厂的理化性质联系起来。通过创建一个具有数千个时间分辨微生物种群的共生网络，这些种群来自于 100 多个样本，跨越了四种运行配置，确定了具有潜在甲烷生产能力的已知和新型微生物群落。通过将宏基因组组装基因组和同源基因表达数据映射到网络上，进一步解析了这些种群与特定工艺配置之间的相互作用。突出的相互作用包括转录活跃的 Methanolinea 产甲烷菌和协同的苯甲酸氧化菌 Syntrophorhabdus，以及 Methanoregulaceae 种群和可能的与拟杆菌门（Tenuifilaceae）相关的协同乙酸盐氧化细菌，它们表达伍德-吕恩达尔途径的甘氨酸裂解旁路。
