Center for Traffic Evidence Technology, Zhengzhou Key Laboratory of Criminal Science and Technology, Department of Criminal Science and Technology, Railway Police College, Zhengzhou, 450053, People's Republic of China.
Sci Rep. 2023 Sep 4;13(1):14526. doi: 10.1038/s41598-023-41860-5.
Herein, a novel Ag NP substrate doped with Au nanobipyramids was designed and fabricated via a convenient procedure of galvanic reaction for the identification and classification of amphetamine-type stimulants (ATS) in oral fluids in combination with surface enhanced Raman scattering (SERS). The substrate was shown to have a three-dimensional nanostructure, high SERS activity, and good stability. In combination with SERS, the Ag NP substrate doped with Au nanobipyramids was able to detect ultra-low traces of ATS, including amphetamine, methylamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethylamphetamine (MDMA) in oral fluid with limit of detection (LOD) and limit of determination quantitation (LOQ) as low as 10 mg/mL, which is much better than the current spectroscopic techniques. The equations between concentration and peaks intensity for quantitative analysis displied good doublelogarithmic linear relations and reliability figures of merit at nanogram concentration level in compartion with GC-MS method. The approach can be broadly applied to the ultra-low trace detection of ATS in oral fluid and would be particularly useful for the analyses of nitrogenous organic compounds.
本文设计并制备了一种新型的金纳米棒掺杂银纳米粒子基底，通过简便的电置换反应将其用于口腔液中苯丙胺类兴奋剂（ATS）的识别和分类，并结合表面增强拉曼散射（SERS）进行检测。该基底具有三维纳米结构、高 SERS 活性和良好的稳定性。金纳米棒掺杂银纳米粒子基底结合 SERS 可以检测到超痕量的 ATS，包括口腔液中的苯丙胺、甲基苯丙胺（MA）、3,4-亚甲二氧基苯丙胺（MDA）和 3,4-亚甲二氧基甲基苯丙胺（MDMA），检测限（LOD）和定量限（LOQ）低至 10mg/mL，优于目前的光谱技术。与 GC-MS 方法相比，定量分析中浓度与峰强度之间的方程在纳克浓度水平上表现出良好的双对数线性关系和可靠的品质因数。该方法可广泛应用于口腔液中 ATS 的超低痕量检测，特别适用于含氮有机化合物的分析。
