School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.
Int J Nanomedicine. 2023 Aug 30;18:4885-4906. doi: 10.2147/IJN.S422944. eCollection 2023.
Deaths from bacterial infections have risen year by year. This trend is further aggravated as the overuse antibiotics and the bacterial resistance to all known antibacterial agents. Therefore, new therapeutic alternatives are urgently needed.
Enlightenment the combination usage of traditional herb medicine, one carrier-free binary nanoparticles (GA-BBR NPs) was discovered, which was self-assembled from gallic acid and berberine through electrostatic interaction, π-π stacking and hydrophobic interaction; and it could be successfully prepared by a green, cost-effective and "one-pot" preparation process.
The nanoparticles exhibited strong antibacterial activity and biofilm removal ability against multidrug-resistant (MRSA) by downregulating mRNA expression of and to block bacterial translation mechanisms in vitro and in vivo, and it had well anti-inflammatory activity and a promising role in promoting angiogenesis to accelerate the wound healing on MRSA-infected wounds model in vivo. Additionally, the nanoparticles displayed well biocompatibility without cytotoxicity, hemolytic activity, and tissue or organ toxicity.
GA-BBR NPs originated from the drug combination has potential clinical transformation value, and this study provides a new idea for the design of carrier-free nanomedicine derived from natural herbals.
细菌感染导致的死亡人数逐年上升。由于抗生素的过度使用和细菌对所有已知抗菌药物的耐药性，这一趋势进一步加剧。因此，迫切需要新的治疗方法。
受传统草药联合使用的启发，我们发现了一种无载体的二元纳米粒子（GA-BBR NPs），它是通过静电相互作用、π-π 堆积和疏水相互作用自组装而成的，由没食子酸和小檗碱组成；并可以通过绿色、经济高效的“一锅”制备工艺成功制备。
该纳米粒子在体外和体内通过下调 和 的 mRNA 表达，具有很强的抗多药耐药性金黄色葡萄球菌（MRSA）的抗菌活性和生物膜去除能力，从而阻断细菌的翻译机制，同时具有良好的抗炎活性，并有望通过促进血管生成来加速 MRSA 感染伤口模型体内的伤口愈合。此外，该纳米粒子表现出良好的生物相容性，无细胞毒性、溶血活性、组织或器官毒性。
源于药物联合的 GA-BBR NPs 具有潜在的临床转化价值，本研究为源自天然草药的无载体纳米药物的设计提供了新的思路。
