Department of Chemistry, Government Postgraduate College No. 1, Abbottabad, 22010, KPK, Pakistan.
Department of Higher Education Archives and Library, Government of Khyber Pakhtunkhwa, Peshawar, Pakistan.
Sci Rep. 2024 Nov 9;14(1):27415. doi: 10.1038/s41598-024-79121-8.
Efficient wound and burn healing is crucial for minimising complications, preventing infections, and enhancing overall well-being, necessitating the development of innovative strategies. This study aimed to formulate a novel thin film combining chitosan, carboxymethyl cellulose, tannic acid, and beeswax for improved wound healing applications. Several formulations, incorporating chitosan, carboxymethyl cellulose, tannic acid, and beeswax in various percentages, were utilized to deposit thin films via the solvent evaporation technique, Mechanical properties, morphology, antioxidant activity, antibacterial efficacy, and wound healing potential were evaluated. The optimized thin film (M4), composed of 2% chitosan, 2% carboxymethyl cellulose, and 1% tannic acid, along with 0.2% glycerol and 0.2% tween80, exhibited a thickness of 39.0 ± 1.14 μm and a tensile strength of 0.275 ± 0.003 MPa. It demonstrated a swelling degree of 283.0 ± 2.0% and a drug release capacity of 89.4% within 24 h. The film also showed a low contact angle of 40.5° and a water vapour transmission rate of 1912.25 ± 13.10 g m 0.24 h. FT-IR spectroscopy indicated that chitosan and carboxymethyl cellulose were cross-linked through amide linkages, with tannic acid occupying the interstitial spaces and hydrogen bonding stabilizing the structure. Microscopy of M4 revealed a uniform morphology. The film exhibited strong antioxidant activity of (95.17 ± 0.02%) and antibacterial efficiency (80.8%) against S. aureus. In a rabbit model, the film significantly enhanced burn and excision wound recovery, with 90.0 ± 3.3% healing for burns and 88.85 ± 1.7% for infected wounds by day 7. Complete skin regeneration was observed within 10-12 days. The M4 thin film demonstrated exceptional mechanical properties and bioactivity, offering protection against pathogens and promoting efficient wound healing. These findings suggest its potential for further investigation in treating various infections and its role in developing novel therapeutic interventions.
高效的伤口和烧伤愈合对于减少并发症、预防感染和提高整体健康至关重要，这需要开发创新的策略。本研究旨在制备一种新型的壳聚糖、羧甲基纤维素、鞣酸和蜂蜡复合薄膜，以提高伤口愈合应用。通过溶剂蒸发技术，利用几种包含壳聚糖、羧甲基纤维素、鞣酸和蜂蜡的配方来沉积薄膜。评估了机械性能、形态、抗氧化活性、抗菌功效和伤口愈合潜力。由 2%壳聚糖、2%羧甲基纤维素和 1%鞣酸组成的优化薄膜（M4），同时含有 0.2%甘油和 0.2%吐温 80，其厚度为 39.0±1.14μm，拉伸强度为 0.275±0.003MPa。它的溶胀度为 283.0±2.0%，在 24 小时内药物释放量为 89.4%。该薄膜还具有低接触角 40.5°和水蒸气传输率 1912.25±13.10g m 0.24 h。傅里叶变换红外光谱表明壳聚糖和羧甲基纤维素通过酰胺键交联，鞣酸占据间隙，氢键稳定结构。M4 的显微镜观察显示出均匀的形态。该薄膜表现出很强的抗氧化活性（95.17±0.02%）和抗菌效率（80.8%）对金黄色葡萄球菌。在兔模型中，该薄膜显著促进烧伤和切除伤口的恢复，烧伤的愈合率为 90.0±3.3%，感染伤口的愈合率为 7 天的 88.85±1.7%。在 10-12 天内观察到完全的皮肤再生。M4 薄膜表现出优异的机械性能和生物活性，提供对病原体的保护并促进有效的伤口愈合。这些发现表明它在治疗各种感染方面有进一步研究的潜力，并在开发新的治疗干预措施方面发挥作用。
