Lu Dingqi, Zhu Xinchao, Hong Tao, Yao Xinyi, Xie Zhiming, Chen Liying, Wang Yihan, Zhang Kaiyuan, Ren Yating, Cao Yi, Wang Xinchang
First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 310053, People's Republic of China.
Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 310053, People's Republic of China.
J Inflamm Res. 2023 Aug 30;16:3811-3822. doi: 10.2147/JIR.S426337. eCollection 2023.
Systemic lupus erythematosus is a heterogeneous autoimmune disease in which skin involvement is a common manifestation. It is currently thought that the photosensitivity of SLE skin involvement is associated with anti-SSA antibodies. This study aimed to expand the current state of knowledge surrounding the molecular pathophysiology of SLE skin photosensitivity through Serum metabolomics analysis.
The serum metabolites of 23 cases of skin-involved SLE (SI) group, 14 cases of no SI (NSI) group, and 30 cases of healthy controls (HC) were analyzed by using UPLC-MS/MS technology, and subgroup analysis was performed according to the expression of anti-SSA antibodies in SI. MetaboAnalyst 5.0 was used for enrichment analysis and ROC curve construction, identifying serum metabolic markers of skin-involved SLE associated with anti-SSA antibodies.
We identified several metabolites and metabolic pathways associated with SLE photosensitivity. Two metabolites, SM (d18:1/24:0) and gamma-CEHC can distinguish between anti-SSA antibody-positive and negative SI, with AUC of 0.829 and 0.806. These two photosensitization-related substances may be potential markers of skin involvement in SLE associated with anti-SSA antibody.
This study provides new insights into the pathogenesis of SI patients, and provides a new molecular biological basis for the association between anti-SSA antibodies and skin photoallergic manifestations of SLE.
系统性红斑狼疮是一种异质性自身免疫性疾病，皮肤受累是其常见表现。目前认为，系统性红斑狼疮皮肤受累的光敏性与抗SSA抗体有关。本研究旨在通过血清代谢组学分析，拓展关于系统性红斑狼疮皮肤光敏性分子病理生理学的现有知识。
采用超高效液相色谱-串联质谱技术分析23例有皮肤受累的系统性红斑狼疮（SI）组、14例无皮肤受累（NSI）组和30例健康对照（HC）的血清代谢物，并根据SI组中抗SSA抗体的表达进行亚组分析。使用MetaboAnalyst 5.0进行富集分析和ROC曲线构建，确定与抗SSA抗体相关的有皮肤受累的系统性红斑狼疮的血清代谢标志物。
我们鉴定出了几种与系统性红斑狼疮光敏性相关的代谢物和代谢途径。两种代谢物，SM（d18:1/24:0）和γ-CEHC能够区分抗SSA抗体阳性和阴性的SI患者，曲线下面积分别为0.829和0.806。这两种与光敏性相关的物质可能是与抗SSA抗体相关的系统性红斑狼疮皮肤受累的潜在标志物。
本研究为有皮肤受累的系统性红斑狼疮患者的发病机制提供了新的见解，并为抗SSA抗体与系统性红斑狼疮皮肤光过敏表现之间的关联提供了新的分子生物学基础。
