MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
Department of Biochemisty and Systems Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.
IUCrJ. 2023 Sep 1;10(Pt 5):519-520. doi: 10.1107/S2052252523006759.
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding. During the last ten years, cryoEM has become widely available and has provided a major boost to structure determination of membrane proteins and large multi-protein complexes. Many of the structures have now been made available at resolutions around 2 Å, where fundamental questions regarding enzyme mechanisms can be addressed. Over the years, the abbreviation cryoEM has been understood to stand for different things. We wish the wider community to engage and clarify the definition of cryoEM so that the expanding literature involving cryoEM is unified.
结构生物学继续受益于不断扩大的工具包,这有助于前所未有地深入了解多蛋白机器、酶机制和配体/抑制剂结合的组装和组织。在过去的十年中,冷冻电镜已经广泛应用,并为膜蛋白和大型多蛋白复合物的结构测定提供了重大推动。现在,许多结构已经可以达到约 2 Å 的分辨率,在这个分辨率下可以解决有关酶机制的基本问题。多年来,cryoEM 的缩写已经被理解为代表不同的事物。我们希望更广泛的社区参与并澄清 cryoEM 的定义,以便统一涉及 cryoEM 的不断扩展的文献。