Kennedy S W, James C A
Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Quebec.
J Chromatogr. 1993 Sep 8;619(1):127-32. doi: 10.1016/0378-4347(93)80455-d.
A new method to extract and concentrate porphyrins from liver tissue for analysis by high-performance liquid chromatography is described. Porphyrins were extracted with acetonitrile-hydrochloric acid, concentrated on disposable octadecylsilyl columns, brought to dryness, and reconstituted in 1.2 M hydrochloric acid. Dried porphyrins were stable at -80 degrees C for at least three weeks, and porphyrins reconstituted in hydrochloric acid were stable for at least 20 h. Yields of uro-, heptacarboxyl-, hexacarboxyl-, pentacarboxyl- and coproporphyrin were > or = 90%. The method allows determination of porphyrin concentrations in small (10-100 mg) samples of liver tissue.
本文描述了一种从肝脏组织中提取和浓缩卟啉以用于高效液相色谱分析的新方法。用乙腈 - 盐酸提取卟啉，在一次性十八烷基硅柱上浓缩，干燥后再用1.2 M盐酸复溶。干燥的卟啉在 -80℃至少稳定三周，盐酸复溶后的卟啉至少稳定20小时。尿卟啉、七羧基卟啉、六羧基卟啉、五羧基卟啉和粪卟啉的回收率≥90%。该方法可测定小量（10 - 100 mg）肝脏组织样本中的卟啉浓度。
