Department of Neurosurgery, Düzce University, Faculty of Medicine, Düzce, Turkey.
Department of Histology and Embryology, Düzce University, Faculty of Medicine, Düzce, Turkey.
Acta Orthop Traumatol Turc. 2023 Jul;57(4):127-133. doi: 10.5152/j.aott.2023.22128.
This study aimed to determine whether isotretinoin and acitretin have beneficial effects on neural tissue damage following acute spinal cord injury.
Thirty-six rats were randomly divided into 6 groups: control, sham spinal cord injury, spinal cord injury with isotretinoin 15 mg/kg for 14 days, spinal cord injury with isotretinoin 15 mg/kg for 28 days, spinal cord injury with acitretin 10 mg/kg for 14 days, and spinal cord injury with acitretin 10 mg/kg for 28 days. The damage to the spinal cord was formed by the clip compression technique. A neurological evaluation was conducted on days 1, 14, and 28. All rats were sacrificed following the treatment period, and samples of their spinal cords were collected for histopathological analysis.
The inclined plane angle was significantly increased on the 14th and 28th days in the isotretinoin 15 mg and acitretin 10 mg groups, compared to the spinal injury group (P=.049 and P=.009, respectively). The Drummond-Moore criterion was significantly higher in the acitretin 10 mg group than in the injury group (P=.026). Cleaved Caspase-3 expression was similar in the isotretinoin 15 mg day 28 group and the control group (P > .05), but significantly decreased in the acitretin 10 mg 14th-day and acitretin 10 mg 28th-day groups compared to spinal injury isotretinoin 15 mg 14th-day and isotretinoin 15 mg 28th-day groups (P < .05).
This was the first study elaborating that isotretinoin and acitretin reduced neuronal apoptosis and improved functional recovery after spinal cord injury. These neuroprotective effects might open a window of opportunity for patients.
本研究旨在确定异维 A 酸和阿维 A 酯是否对急性脊髓损伤后的神经组织损伤有有益作用。
36 只大鼠随机分为 6 组：对照组、假脊髓损伤组、脊髓损伤后给予异维 A 酸 15mg/kg 治疗 14 天组、脊髓损伤后给予异维 A 酸 15mg/kg 治疗 28 天组、脊髓损伤后给予阿维 A 酯 10mg/kg 治疗 14 天组和脊髓损伤后给予阿维 A 酯 10mg/kg 治疗 28 天组。采用夹压技术造成脊髓损伤。在第 1、14 和 28 天进行神经学评估。治疗期结束后，所有大鼠均被处死，收集其脊髓样本进行组织病理学分析。
在异维 A 酸 15mg 和阿维 A 酯 10mg 组，第 14 天和第 28 天的斜面角度明显增加，与脊髓损伤组相比（分别为 P=.049 和 P=.009）。阿维 A 酯 10mg 组的 Drummond-Moore 评分明显高于损伤组（P=.026）。第 28 天异维 A 酸 15mg 组的 cleaved Caspase-3 表达与对照组相似（P>.05），但在阿维 A 酯 10mg 第 14 天和阿维 A 酯 10mg 第 28 天组与脊髓损伤异维 A 酸 15mg 第 14 天和异维 A 酸 15mg 第 28 天组相比明显降低（P<.05）。
这是第一项详细阐述异维 A 酸和阿维 A 酯减少神经元凋亡并改善脊髓损伤后功能恢复的研究。这些神经保护作用可能为患者提供机会。
