Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Talanta. 2024 Jan 15;267:125150. doi: 10.1016/j.talanta.2023.125150. Epub 2023 Sep 3.
Numerous diseases have posed significant threats to public health, notably the global pandemic of COVID-19, resulting in widespread devastation due to its high infectivity and severity. The nucleic acid lateral flow assay (NALFA) addresses challenges of complexity, cost, and time associated with traditional assays, offering a reliable platform for rapid and precise nucleic acid target detection. NALFA is gaining prominence as a point-of-care testing (POCT) technique, thanks to its user-friendly operation and rapid results. Nevertheless, conventional NALFA relies on specialized technicians and involves labor-intensive steps like DNA extraction and PCR processes, impeding its efficiency. To overcome these limitations, integrating NALFA with microfluidic technology, widely employed in rapid field detection, holds promise. This review comprehensively outlines prevailing strategies for integrating NALFA, encompassing both research initiatives and commercial applications. Addressing the bottleneck of nucleic acid amplification as a rate-limiting step, the review delves into progress in amplification-free NALFA and highlights prevalent signal amplification techniques. Ultimately, the review outlines the future prospect of integrated NALFA development, capturing the technology's evolution and providing valuable insights for academic and commercial endeavors.
许多疾病对公众健康构成了重大威胁，尤其是 COVID-19 全球大流行，由于其高传染性和严重性，造成了广泛的破坏。核酸侧向流动检测（NALFA）解决了传统检测方法复杂、成本高和耗时的问题，为快速、准确地检测核酸靶标提供了可靠的平台。NALFA 作为一种即时检测（POCT）技术越来越受到关注，因为它操作简单，结果快速。然而，传统的 NALFA 依赖于专业技术人员，并且涉及到 DNA 提取和 PCR 等劳动密集型步骤，这限制了它的效率。为了克服这些限制，将 NALFA 与微流控技术结合，微流控技术广泛应用于快速现场检测，具有广阔的前景。本综述全面概述了将 NALFA 集成的现有策略，包括研究计划和商业应用。该综述解决了作为限速步骤的核酸扩增的瓶颈问题，深入探讨了无扩增 NALFA 的进展，并强调了常见的信号放大技术。最终，该综述概述了集成 NALFA 发展的未来前景，捕捉了该技术的演变，并为学术和商业努力提供了有价值的见解。
