Xu Yuan, Freund Dana M, Hegeman Adrian D, Cohen Jerry D
Department of Horticultural Science and the Microbial and Plant Genomics Institute, University of Minnesota, MN, Saint Paul, USA.
Department of Plant and Microbial Biology, University of Minnesota, MN, Saint Paul, USA.
Stress Biol. 2022 Feb 15;2(1):11. doi: 10.1007/s44154-022-00034-5.
Temperature, water, and light are three abiotic stress factors that have major influences on plant growth, development, and reproduction. Plants can be primed by a prior mild stress to enhance their resistance to future stress. We used an untargeted metabolomics approach to examine Arabidopsis thaliana 11-day-old seedling's abiotic stress responses including heat (with and without priming), cold (with and without priming), water-deficit and high-light before and after a 2-day-recovery period. Analysis of the physiological phenotypes showed that seedlings with stress treatment resulted in a reduction in fresh weight, hypocotyl and root length but remained viable. Several stress responsive metabolites were identified, confirmed with reference standards, quantified, and clustered. We identified shared and specific stress signatures for cold, heat, water-deficit, and high-light treatments. Central metabolism including amino acid metabolism, sugar metabolism, glycolysis, TCA cycle, GABA shunt, glutathione metabolism, purine metabolism, and urea cycle were found to undergo changes that are fundamentally different, although some shared commonalities in response to different treatments. Large increases in cysteine abundance and decreases in reduced glutathione were observed following multiple stress treatments highlighting the importance of oxidative stress as a general phenomenon in abiotic stress. Large fold increases in low-turnover amino acids and maltose demonstrate the critical role of protein and starch autolysis in early abiotic stress responses.
温度、水分和光照是对植物生长、发育和繁殖有重大影响的三个非生物胁迫因素。植物可以通过预先的轻度胁迫进行预处理，以增强其对未来胁迫的抗性。我们采用非靶向代谢组学方法，研究了拟南芥11日龄幼苗在2天恢复期前后对包括热胁迫（有和无预处理）、冷胁迫（有和无预处理）、水分亏缺和高光胁迫在内的非生物胁迫的响应。生理表型分析表明，经过胁迫处理的幼苗鲜重、下胚轴和根长减少，但仍能存活。鉴定了几种胁迫响应代谢物，用参考标准品进行了确认、定量和聚类。我们确定了冷胁迫、热胁迫、水分亏缺和高光处理的共同和特定胁迫特征。发现包括氨基酸代谢、糖代谢、糖酵解、三羧酸循环、γ-氨基丁酸分流、谷胱甘肽代谢、嘌呤代谢和尿素循环在内的中心代谢发生了根本不同的变化，尽管在对不同处理的响应中存在一些共同之处。多次胁迫处理后，半胱氨酸丰度大幅增加，还原型谷胱甘肽减少，突出了氧化应激作为非生物胁迫中的普遍现象的重要性。低周转率氨基酸和麦芽糖的大幅增加表明蛋白质和淀粉自溶在早期非生物胁迫响应中的关键作用。
