Redlich J, Souffrant W B, Laplace J P, Hennig U, Berg R, Mouwen J M
Institute für Veterinär-Anatomie, Freien Universität Berlin, Germany.
Can J Vet Res. 1997 Jan;61(1):21-7.
Ileo-rectal anastomosis (IRA), which is frequently used to measure prececal digestibility in pigs, could induce some disturbances of the normal absorptive function. Our aim was to investigate the effects of different IRA surgical procedures on the main histologic characteristics of the small intestine in pigs. The 4 different IRA procedures compared to intact pigs (INT) were the following: either end to end (EE) or end to side (ES) with or without preservation of the ileocecal valve (EEV, EE, ESV, ES respectively). At 147 d after surgery, samples of the wall of the duodenum, jejunum and ileum were taken under anesthesia and histometric examinations were performed on HE- and PAS-colored sections to estimate changes mainly of mucosa and muscle layers. The values recorded for villus length, crypt depth, and whole thickness of the mucosa suggested that the EE procedures disturb the small intestine less than the ES models. A new parameter, called epithelial quotient and calculated as [(villus length/crypt depth)/mitotic index], was proposed to improve the comparisons. According to this quotient, EE procedures did not significantly affect the mucosa of the whole small intestine. An increased density of goblet cells was recorded in all operated pigs along the small intestine, but mainly in the ileum after EE-IRA. The lymphatic follicle area was reduced. These findings, which were in agreement with a reduced mitotic index in the ileum of EE-pigs, indicated a decreased effect of noxious factors on the small intestinal mucosa in IRA-pigs, especially after the EE-IRA procedure. Some atrophic or hypertrophic effects on the muscle layers were related to the absence or preservation of the ileo-cecal valve. Finally it was concluded that i) there was no major disturbance after IRA, and ii) the end to end procedure was most beneficial for the structural integrity of the small intestine.
回肠直肠吻合术（IRA）常用于测定猪的盲肠前消化率，但可能会引起正常吸收功能的一些紊乱。我们的目的是研究不同的IRA手术操作对猪小肠主要组织学特征的影响。与完整猪（INT）相比，所比较的4种不同IRA手术操作如下：端端吻合（EE）或端侧吻合（ES），保留或不保留回盲瓣（分别为EEV、EE、ESV、ES）。术后147天，在麻醉下采集十二指肠、空肠和回肠壁样本，并对苏木精-伊红（HE）染色和过碘酸-希夫（PAS）染色切片进行组织测量检查，以评估主要是黏膜层和肌层的变化。绒毛长度、隐窝深度和黏膜全层厚度的记录值表明，EE手术操作对小肠的干扰小于ES模型。提出了一个新参数，称为上皮商，计算方法为[（绒毛长度/隐窝深度）/有丝分裂指数]，以改进比较。根据这个商值，EE手术操作对整个小肠黏膜没有显著影响。在所有手术猪的小肠中，杯状细胞密度增加，但主要是在EE-IRA术后的回肠中。淋巴滤泡面积减小。这些发现与EE猪回肠中有丝分裂指数降低一致，表明IRA猪小肠黏膜上有害因素的影响降低，尤其是在EE-IRA手术后。对肌层的一些萎缩或肥大效应与回盲瓣的缺失或保留有关。最后得出结论：i）IRA术后没有重大干扰，ii）端端吻合术对小肠的结构完整性最有益。
