State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Centre for New Organic Matter, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry and School of Medicine, Nankai University, Tianjin, 300071, China.
Qingdao Academy of Agricultural Sciences, Qingdao, 266100, China.
Biosens Bioelectron. 2023 Nov 15;240:115649. doi: 10.1016/j.bios.2023.115649. Epub 2023 Aug 31.
Lipids are important molecules that are widely distributed within the cell, and they play a crucial role in several biological processes such as cell membrane formation, signaling, cell motility and division. Monitoring the spatiotemporal dynamics of cellular lipids in real-time and quantifying their concentrations in situ is crucial since the local concentration of lipids initiates various signaling pathways that regulate cellular processes. In this review, we first introduced the historical background of lipid quantification methods. We then delve into the current state of the art of in situ lipid quantification, including the establishment and utility of fluorescence imaging techniques based on sensors of lipid-binding domains labeled with organic dyes or fluorescent proteins, and Raman and magnetic resonance imaging (MRI) techniques that do not require lipid labeling. Next, we highlighted the biological applications of live-cell lipid quantification techniques in the study of in situ lipid distribution, lipid transformation, and lipid-mediated signaling pathways. Finally, we discussed the technical challenges and prospects for the development of lipid quantification in live cells, with the aim of promoting the development of in situ lipid quantification in live cells, which may have a profound impact on the biological and medical fields.
脂质是广泛分布于细胞内的重要分子，在细胞膜形成、信号转导、细胞运动和分裂等多种生物学过程中发挥着关键作用。实时监测细胞脂质的时空动力学并原位定量其浓度至关重要，因为脂质的局部浓度会引发各种信号通路，从而调节细胞过程。在这篇综述中，我们首先介绍了脂质定量方法的历史背景。然后，我们深入探讨了原位脂质定量的最新技术，包括基于脂质结合域传感器的荧光成像技术的建立和应用，这些传感器标记有有机染料或荧光蛋白，以及不需要脂质标记的拉曼和磁共振成像（MRI）技术。接下来，我们强调了活细胞脂质定量技术在研究原位脂质分布、脂质转化和脂质介导的信号通路中的生物学应用。最后，我们讨论了活细胞中脂质定量发展的技术挑战和前景，旨在促进活细胞中原位脂质定量的发展，这可能对生物和医学领域产生深远影响。
