State Key Laboratory of Genetic Engineering, School of Life Sciences and Human Phenome Institute, Shanghai Cancer Center, Fudan University, Shanghai, China.
National Institute of Metrology, Beijing, China.
Nat Biotechnol. 2024 Jul;42(7):1118-1132. doi: 10.1038/s41587-023-01867-9. Epub 2023 Sep 7.
Certified RNA reference materials are indispensable for assessing the reliability of RNA sequencing to detect intrinsically small biological differences in clinical settings, such as molecular subtyping of diseases. As part of the Quartet Project for quality control and data integration of multi-omics profiling, we established four RNA reference materials derived from immortalized B-lymphoblastoid cell lines from four members of a monozygotic twin family. Additionally, we constructed ratio-based transcriptome-wide reference datasets between two samples, providing cross-platform and cross-laboratory 'ground truth'. Investigation of the intrinsically subtle biological differences among the Quartet samples enables sensitive assessment of cross-batch integration of transcriptomic measurements at the ratio level. The Quartet RNA reference materials, combined with the ratio-based reference datasets, can serve as unique resources for assessing and improving the quality of transcriptomic data in clinical and biological settings.
用于评估 RNA 测序在临床环境中检测内在生物学差异（如疾病的分子亚型）可靠性的经认证的 RNA 参考材料是不可或缺的。作为 Quartet 项目（用于多组学分析的质量控制和数据集成）的一部分，我们建立了四种源自同卵双胞胎家族的四个成员的永生 B 淋巴细胞样细胞系的 RNA 参考材料。此外，我们构建了两个样本之间基于比值的转录组全基因组参考数据集，提供了跨平台和跨实验室的“真实数据”。对 Quartet 样本之间内在细微生物学差异的研究能够敏感地评估转录组测量在比值水平上的跨批次整合。 Quartet RNA 参考材料与基于比值的参考数据集相结合，可作为评估和改善临床和生物学环境中转录组数据质量的独特资源。
