Department of Genome Sciences, University of Washington, 3720 15th Street NE, Seattle, Washington 98195, United States.
Thermo Fisher Scientific, Hanna-Kunath Ste. 11, 28199 Bremen, Germany.
J Proteome Res. 2023 Oct 6;22(10):3290-3300. doi: 10.1021/acs.jproteome.3c00357. Epub 2023 Sep 8.
We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific Orbitrap mass spectrometers, which have long been the gold standard for high-resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high-quality quantitative measurements across a wide dynamic range. We also use a newly developed extracellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5000 plasma proteins in a 60 min gradient with the Orbitrap Astral mass spectrometer.
我们评估了新发布的不对称轨道无损耗（Astral）分析器的定量性能。使用数据独立采集，赛默飞世尔科技的 Orbitrap Astral 质谱仪在单位时间内定量的肽数量比长期以来一直是高分辨率定量蛋白质组学黄金标准的最先进的赛默飞世尔科技 Orbitrap 质谱仪多 5 倍。我们的结果表明，Orbitrap Astral 质谱仪可以在很宽的动态范围内产生高质量的定量测量结果。我们还使用新开发的细胞外囊泡富集方案，在血浆蛋白质组中达到新的覆盖深度，使用 Orbitrap Astral 质谱仪在 60 分钟梯度中定量超过 5000 种血浆蛋白。
