Kong Ran, Yan Kaixian, Liu Defeng, Wu Wenying
Institute of Advanced Clinical Medicine, Institute of Medical Technology, Peking University Health Science Center, Beijing, China.
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
Sci Data. 2025 Aug 28;12(1):1509. doi: 10.1038/s41597-025-05824-w.
Male infertility is fundamentally rooted in developmental defects of germ cells and associated molecular dysregulation, yet the underlying mechanisms remain poorly understood. Data-independent acquisition (DIA) has emerged as a powerful tool in discovery proteomics, enabling the identification of disease biomarkers, therapeutic targets, and molecular pathways with high precision and reproducibility. To analyse the aberrant regulatory events in human sperm proteins associated with male reproductive disorders in a high-throughput, reproducible, and reliable manner, we employed the Orbitrap Astral mass spectrometer, which independently operates Orbitrap Full Scan and Astral MS/MS to generate high-resolution full-scan spectra and high-quality secondary maps. Leveraging the principles of DIA technology, we constructed the most comprehensive human sperm proteomic expression profile reported to date, encompassing 9,309 proteins, 198,153 unique precursors, 154,062 modified peptides, and 145,355 peptides. Moreover, this data is derived from two types of sperm samples to facilitate the exploration of disease-specific proteins. Our data serve as a valuable resource for analysing spermatozoa protein content, facilitating deeper insights into male reproduction.
男性不育症从根本上源于生殖细胞的发育缺陷和相关分子失调，但其潜在机制仍知之甚少。数据非依赖采集（DIA）已成为发现蛋白质组学中的一种强大工具，能够高精度、可重复地鉴定疾病生物标志物、治疗靶点和分子途径。为了以高通量、可重复和可靠的方式分析与男性生殖障碍相关的人类精子蛋白质中的异常调控事件，我们使用了Orbitrap Astral质谱仪，它独立运行Orbitrap全扫描和Astral MS/MS，以生成高分辨率全扫描光谱和高质量二级图谱。利用DIA技术原理，我们构建了迄今为止报道的最全面的人类精子蛋白质组表达谱，涵盖9309种蛋白质、198153个独特前体、154062个修饰肽和145355个肽段。此外，这些数据来自两种类型的精子样本，以促进对疾病特异性蛋白质的探索。我们的数据是分析精子蛋白质含量的宝贵资源，有助于更深入地了解男性生殖。
