Nie Miaomiao, Tan Xungang, Lu Yunliang, Wu Zhihao, Li Jun, Xu Dongdong, Zhang Peijun, You Feng
CAS Key Laboratory of Experimental Marine Biology, National and Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People's Republic of China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, People's Republic of China.
Fish Physiol Biochem. 2019 Apr;45(2):583-597. doi: 10.1007/s10695-019-00611-y. Epub 2019 Feb 21.
The aim of this study is to understand fish cold-tolerant mechanism. We analyzed the transcriptional reactions to the cold condition in turbot Scophthalmus maximus by using RNA-seq and microRNA (miRNA)-seq. Meio-gynogenetic diploid turbots were treated at 0 °C to distinguish the cold-tolerant (CT) and cold-sensitive (CS) groups. The results showed that there were quite different responses at both mRNA and miRNA levels, with more up-regulated mRNAs (1069 vs. 194) and less down-regulated miRNAs (4 vs. 1) in CT versus CS relative to the control group. The network of miRNA-transcription factor-mRNA, regulating turbot different response to cold stress, was constructed, which involved in cell cycle, component of cell membrane, signal transduction, and circadian rhythm pathways. The above information demonstrates mechanisms by which cold tolerance is increased in fish.
本研究的目的是了解鱼类的耐寒机制。我们通过RNA测序和微小RNA（miRNA）测序分析了大菱鲆（Scophthalmus maximus）对寒冷条件的转录反应。对雌核发育二倍体大菱鲆在0°C下进行处理，以区分耐寒（CT）组和冷敏感（CS）组。结果表明，在mRNA和miRNA水平上均有相当不同的反应，相对于对照组，CT组中上调的mRNA更多（1069对194），下调的miRNA更少（4对1）。构建了调节大菱鲆对冷应激不同反应的miRNA-转录因子-mRNA网络，其涉及细胞周期、细胞膜成分、信号转导和昼夜节律途径。上述信息揭示了鱼类耐寒性增强的机制。
