Mou Lu, Pan Runze, Liu Yansong, Jiang Wankui, Zhang Wenming, Jiang Yujia, Xin Fengxue, Jiang Min
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China; Jiangsu Academy of Chemical Inherent Safety, Nanjing 211800, PR China.
Enzyme Microb Technol. 2023 Dec;171:110318. doi: 10.1016/j.enzmictec.2023.110318. Epub 2023 Sep 4.
As the most abundant and renewable natural resource in the world, lignocellulose is a promising alternative to fossil energy to relieve environmental concerns and resource depletion. However, due to its recalcitrant structure, strains with efficient degradation capability still need exploring. In this study, a fungus was successfully isolated from decayed wood and named as Trichoderma asperellum LYS1 by phylogenetic and draft genomic analysis. The further investigations showed that strain LYS1 had an outstanding performance on lignocellulose degradation, especially for hemicellulose-rich biomass. After the analysis of encoded CAZymes, mainly on GH family, a large amount of genes coding β-glucosidase and xylanase may contribute to the high degradation of cellulose and hemicellulose. Collectively, the results generated in this study demonstrated that T. asperellum LYS1 is a potential cell factory for lignocellulose biorefinery.
作为世界上最丰富且可再生的自然资源，木质纤维素是一种有前景的替代化石能源的物质，有助于缓解环境问题和资源枯竭。然而，由于其顽固的结构，仍需探索具有高效降解能力的菌株。在本研究中，从腐朽木材中成功分离出一种真菌，通过系统发育和基因组草图分析将其命名为棘孢木霉LYS1。进一步研究表明，菌株LYS1在木质纤维素降解方面表现出色，尤其是对富含半纤维素的生物质。在对编码的碳水化合物活性酶（主要是糖苷水解酶家族）进行分析后发现，大量编码β-葡萄糖苷酶和木聚糖酶的基因可能有助于纤维素和半纤维素的高降解率。总体而言，本研究结果表明棘孢木霉LYS1是木质纤维素生物炼制的潜在细胞工厂。
