Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, People's Republic of China.
Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, People's Republic of China; Yunnan University of Chinese Medicine, Kunming 650091, People's Republic of China.
Fitoterapia. 2023 Dec;171:105668. doi: 10.1016/j.fitote.2023.105668. Epub 2023 Sep 6.
The fungus Nectria sp. MHHJ-3 was isolated from Illigera rhodantha. A molecular networking-guided the secondary metabolites investigation of Nectria sp. MHHJ-3 led to the isolation of ten metabolites (1-10), including two new naphthalenone derivatives, nectrianaphthalenones A (1) and B (2), and two new steroids, nectriasteroids A (3) and B (4). Their structures were elucidated by extensive spectroscopic analysis including the HRESIMS, 1D/2D NMR and electronic circular dichroism (ECD) spectra. A plausible biosynthetic pathway for 1-2 was proposed. Compounds 1 and 2 exhibited moderate acetylcholinesterase (AChE) inhibitory activities. Compounds 3 and 4 showed significant cytotoxic activity against selected tumor cells. Particularly, compound 3 exhibited the strongest activity against A549 cells with an IC value of 13.73 ± 0.03 μM, which was at the same grade with that of positive control cisplatin.
从Illigera rhodantha 中分离到真菌 Nectria sp. MHHJ-3。采用分子网络导向的次级代谢产物研究方法，对 Nectria sp. MHHJ-3 进行研究，分离得到了 10 种代谢产物（1-10），其中包括两种新的萘醌衍生物，nectrianaphthalenones A（1）和 B（2），以及两种新的甾体化合物，nectriasteroids A（3）和 B（4）。通过广泛的光谱分析，包括高分辨质谱（HRESIMS）、1D/2D NMR 和电子圆二色谱（ECD）谱，确定了它们的结构。提出了 1-2 的可能生物合成途径。化合物 1 和 2 表现出中等的乙酰胆碱酯酶（AChE）抑制活性。化合物 3 和 4 对选定的肿瘤细胞表现出显著的细胞毒性。特别是化合物 3 对 A549 细胞表现出最强的活性，IC 值为 13.73±0.03 μM，与阳性对照顺铂相当。
