Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Int J Mol Sci. 2023 Aug 24;24(17):13176. doi: 10.3390/ijms241713176.
The oriental river prawn () is a commercially important species in Asia. A previous study showed that the succinate dehydrogenase complex iron sulfur subunit B () gene participates in testes development in this species through its effect on the expression of the insulin-like androgenic gland hormone gene. This study knocked-down the genes in using RNAi. A transcriptome analysis of the androgenic gland and testes was then performed to discover the male sex-related genes regulated by and investigate the mechanism of male sexual development in this species. More than 16,623 unigenes were discovered in each sample generated. In the androgenic gland, most of the differentially expressed genes were enriched in the hypertrophic cardiomyopathy pathway, while in the testes, they were enriched in the citrate cycle pathway. In addition, after knockdown, five genes were found to be downregulated in the androgenic gland in a series of biological processes associated with phosphorylated carbohydrate synthesis and transformations in the glycolysis/gluconeogenesis pathway. Moreover, a total of nine male sex-related genes were identified including Pro-resilin, insulin-like androgenic gland hormone, Protein mono-ADP-ribosyltransferase PAPR11, DNAJC2, C-type Lectin-1, Tyrosine-protein kinase Yes, Vigilin, and Sperm motility kinase Y-like, demonstrating the regulatory effects of , and providing a reference for the further study of the mechanisms of male development in .
东方沼虾（）是亚洲具有商业重要性的物种。先前的研究表明，琥珀酸脱氢酶复合体铁硫亚基 B（）基因通过对胰岛素样促性腺激素基因的表达的影响，参与该物种的精巢发育。本研究通过 RNAi 敲低了 在 中的表达。然后对精巢和输精管进行转录组分析，以发现受 调节的雄性相关基因，并研究该物种雄性性发育的机制。在每个生成的样本中，发现了超过 16623 个基因。在输精管中，大多数差异表达的基因富集在肥厚性心肌病途径中，而在精巢中，它们富集在柠檬酸循环途径中。此外，在 敲低后，发现五个基因在与磷酸化碳水化合物合成和糖酵解/糖异生途径中转化相关的一系列生物学过程中在输精管中下调。此外，总共鉴定出 9 个与雄性相关的基因，包括 Pro-resilin、胰岛素样促性腺激素、蛋白单 ADP-核糖基转移酶 PAPR11、DNAJC2、C 型凝集素-1、酪氨酸蛋白激酶 Yes、Vigilin 和精子运动激酶 Y 样，证明了 的调节作用，并为进一步研究该物种的雄性发育机制提供了参考。
