Xu Ke, Zhao Yong, Zhao Sihang, Liu Haodong, Wang Weiwei, Zhang Shuhua, Yang Xueju
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, China.
Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, China.
Front Plant Sci. 2021 Feb 24;12:618984. doi: 10.3389/fpls.2021.618984. eCollection 2021.
The actin depolymerizing factor (ADF) gene family, which is conserved in eukaryotes, is important for plant development, growth, and stress responses. Cold stress restricts wheat growth, development, and distribution. However, genome-wide identification and functional analysis of the ADF family in wheat is limited. Further, because of the promising role of ADF genes in cold response, there is need for an understanding of the function of this family on wheat under cold stress. In this study, 25 ADF genes () were identified in the wheat genome and they are distributed on 15 chromosomes. The TaADF gene structures, duplication events, encoded conversed motifs, and -acting elements were investigated. Expression profiles derived from RNA-seq data and real-time quantitative PCR analysis revealed the tissue- and temporal-specific TaADF expression patterns. In addition, the expression levels of were significantly affected by cold acclimation or freezing conditions. Overexpression of increased the freezing tolerance of transgenic , possibly because of enhanced ROS scavenging and changes to the osmotic regulation in cells. The expression levels of seven cold-responsive genes were up-regulated in the transgenic Arabidopsis plants, regardless of whether the plants were exposed to low temperature. These findings provide fundamental information about the wheat ADF genes and may help to elucidate the regulatory effects of the encoded proteins on plant development and responses to low-temperature stress.
肌动蛋白解聚因子（ADF）基因家族在真核生物中保守，对植物的发育、生长及胁迫响应至关重要。低温胁迫限制了小麦的生长、发育及分布。然而，对小麦中ADF家族进行全基因组鉴定和功能分析的研究还很有限。此外，鉴于ADF基因在低温响应中具有重要作用，有必要了解该家族在低温胁迫下对小麦的功能。在本研究中，从小麦基因组中鉴定出25个ADF基因，它们分布在15条染色体上。对TaADF基因结构、复制事件、编码的保守基序及顺式作用元件进行了研究。基于RNA测序数据和实时定量PCR分析得到的表达谱揭示了TaADF基因在组织和时间上的特异性表达模式。此外，TaADF基因的表达水平受低温驯化或冷冻条件的显著影响。TaADF基因过表达提高了转基因拟南芥的耐冻性，这可能是由于增强了活性氧清除能力以及细胞渗透调节的改变。无论是否暴露于低温下，7个低温响应基因在转基因拟南芥植株中的表达水平均上调。这些发现提供了有关小麦ADF基因的基础信息，并可能有助于阐明其编码蛋白对植物发育和低温胁迫响应的调控作用。
